TY - JOUR
T1 - Helicobacter priori does not require Lewis X or Lewis Y expression to colonize C3H/HeJ mice
AU - Takata, Tohru
AU - El-Omar, Emad
AU - Camorlinga, Margarita
AU - Thompson, Stuart A.
AU - Minohara, Yutaka
AU - Ernst, Peter B.
AU - Blaser, Martin J.
PY - 2002
Y1 - 2002
N2 - Helicobacter pylori strains frequently express Lewis X (Lex) and/or Ley on their cell surfaces as constituents of the O antigens of their lipopolysaccharide molecules. To assess the effect of Lex and Ley expression on the ability of H. pylori to colonize the mouse stomach and to adhere to epithelial cells, isogenic mutants were created in which fucT1 alone or fucT1 and fucT2, which encode the fucosyl transferases necessary for Lex and Ley expression, were deleted. C3H/HeJ mice were experimentally challenged with either wild-type 26695 H. pylori or its isogenic mutants. All strains, whether passaged in the laboratory or recovered after mouse passage, colonized the mice well and without consistent differences. During colonization by the mutants, there was no reversion to wild type. Similarly, adherence to AGS and KatoIII cells was unaffected by the mutations. Together, these findings indicate that Le expression is not necessary for mouse gastric colonization or for H. pylori adherence to epithelial cells.
AB - Helicobacter pylori strains frequently express Lewis X (Lex) and/or Ley on their cell surfaces as constituents of the O antigens of their lipopolysaccharide molecules. To assess the effect of Lex and Ley expression on the ability of H. pylori to colonize the mouse stomach and to adhere to epithelial cells, isogenic mutants were created in which fucT1 alone or fucT1 and fucT2, which encode the fucosyl transferases necessary for Lex and Ley expression, were deleted. C3H/HeJ mice were experimentally challenged with either wild-type 26695 H. pylori or its isogenic mutants. All strains, whether passaged in the laboratory or recovered after mouse passage, colonized the mice well and without consistent differences. During colonization by the mutants, there was no reversion to wild type. Similarly, adherence to AGS and KatoIII cells was unaffected by the mutations. Together, these findings indicate that Le expression is not necessary for mouse gastric colonization or for H. pylori adherence to epithelial cells.
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U2 - 10.1128/IAI.70.6.3073-3079.2002
DO - 10.1128/IAI.70.6.3073-3079.2002
M3 - Article
C2 - 12011000
AN - SCOPUS:0036014859
SN - 0019-9567
VL - 70
SP - 3073
EP - 3079
JO - Infection and Immunity
JF - Infection and Immunity
IS - 6
ER -