TY - JOUR
T1 - Hormone-independent ovarian influence on adhesion development
AU - Freeman, Michael L.
AU - Saed, Ghassan M.
AU - Diamond, Michael P.
N1 - Funding Information:
Supported in part by a grant from ACOG/Ethicon’s 2001–2002 Research Award for Innovations in Gynecologic Surgery.
PY - 2002
Y1 - 2002
N2 - Objective: To determine which ovarian sex steroid(s), when removed from an intact organism, reduce(s) postoperative adhesion development. Design: Randomized, prospective, blinded study. Setting: University vivarium. Patient(s): One hundred twenty sexually mature female Sprague-Dawley rats, 226-250 g. Intervention(s): Day 0, sham ovariectomy or bilateral ovariectomy, accompanied by continuous-release sex steroid replacement of either no steroids (control), 17β-E2, natural P (P4), or combined E2/P4. Day 7, standardized cecal abrasion; day 14, necropsy with assessment of adhesion presence or absence. Main Outcome Measure(s): Adhesion formation. Result(s): Three rats died because of anesthesia or surgical complications, and 117 rats reached necropsy. The ovary-intact (sham) rats adhesion incidence was 60.9%; ovariectomized control rats, 20.8%; E2, 28.6%; P4, 33.3%; and combined E2/P4, 24.0%. Despite differing sex steroid replacement, two-tailed χ2 testing with correction for multiple comparisons showed no statistical difference in adhesion incidence among the four ovariectomy groups. A statistically significant lower adhesion incidence was noted between the ovary-intact sham cohort and the collective ovariectomy groups and between the sham and ovariectomized control cohorts. Conclusion(s): Ovarian presence or absence at the time of surgical wounding, and not the 17β-E2 or P milieu, modulates adhesion development. This implicates other ovarian factor(s) in postoperative adhesion development.
AB - Objective: To determine which ovarian sex steroid(s), when removed from an intact organism, reduce(s) postoperative adhesion development. Design: Randomized, prospective, blinded study. Setting: University vivarium. Patient(s): One hundred twenty sexually mature female Sprague-Dawley rats, 226-250 g. Intervention(s): Day 0, sham ovariectomy or bilateral ovariectomy, accompanied by continuous-release sex steroid replacement of either no steroids (control), 17β-E2, natural P (P4), or combined E2/P4. Day 7, standardized cecal abrasion; day 14, necropsy with assessment of adhesion presence or absence. Main Outcome Measure(s): Adhesion formation. Result(s): Three rats died because of anesthesia or surgical complications, and 117 rats reached necropsy. The ovary-intact (sham) rats adhesion incidence was 60.9%; ovariectomized control rats, 20.8%; E2, 28.6%; P4, 33.3%; and combined E2/P4, 24.0%. Despite differing sex steroid replacement, two-tailed χ2 testing with correction for multiple comparisons showed no statistical difference in adhesion incidence among the four ovariectomy groups. A statistically significant lower adhesion incidence was noted between the ovary-intact sham cohort and the collective ovariectomy groups and between the sham and ovariectomized control cohorts. Conclusion(s): Ovarian presence or absence at the time of surgical wounding, and not the 17β-E2 or P milieu, modulates adhesion development. This implicates other ovarian factor(s) in postoperative adhesion development.
KW - Abrasion
KW - Adhesions
KW - Cecum
KW - Estradiol
KW - Ovariectomy
KW - Pellets
KW - Progesterone
KW - Rat
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U2 - 10.1016/S0015-0282(02)03237-5
DO - 10.1016/S0015-0282(02)03237-5
M3 - Article
C2 - 12137872
AN - SCOPUS:0036326654
SN - 0015-0282
VL - 78
SP - 340
EP - 346
JO - Fertility and sterility
JF - Fertility and sterility
IS - 2
ER -