Hybrid Root SEAL (MetaSEAL) creates hybrid layers in radicular dentin only when EDTA is used as the final rinse

Luigi Pinna, Robert J. Loushine, Frank D. Bishop, Elisabetta Cotti, R. Norman Weller, David H. Pashley, Franklin R. Tay

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Purpose: To test if the hybrid layer formation by Hybrid Root SEAL (Sun Medical Co.), a 4-META-containing auto-adhesive self-etching root canal sealer, is affected by the sequence of irrigants employed for removing canal wall smear layers during root canal treatment. Methods: Single-rooted teeth were shaped and irrigated with EDTA as initial rinse/NaOCl as active final rinse (Group 1), or NaOCl as initial rinse/EDTA as active final rinse (Group 2). All canals were obturated with Hybrid Root SEAL using a single-cone technique. Root slices derived from the coronal, middle and apical thirds of the roots were processed for transmission electron microscopy after removing the gutta-percha, leaving the sealer intact. Additional filled canals from the two groups were evaluated for fluid leakage. Results: Hybrid layer was absent in Group 1 and was present only when a collagen matrix was produced by EDTA demineralization (Group 2). Significantly more leakage (4.03 ± 1.94 μLmin-1 vs. 1.50 ± 0.42 μLmin-1 P< 0.05) was observed in the absence of dentin hybridization.

Original languageEnglish (US)
Pages (from-to)299-303
Number of pages5
JournalAmerican journal of dentistry
Issue number5
StatePublished - Oct 2009

ASJC Scopus subject areas

  • Dentistry(all)


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