Hydrogen peroxide regulates extracellular superoxide dismutase activity and expression in neonatal pulmonary hypertension

Stephen Wedgwood, Satyan Lakshminrusimha, Tohru Fukai, James A. Russell, Paul T. Schumacker, Robin H. Steinhorn

Research output: Contribution to journalArticlepeer-review

58 Scopus citations


We previously demonstrated that superoxide and H 2O 2 promote pulmonary arterial vasoconstriction in a lamb model of persistent pulmonary hypertension of the newborn (PPHN). Because extracellular superoxide dismutase (ecSOD) augments vasodilation, we hypothesized that H 2O 2-mediated ecSOD inactivation contributes to pulmonary arterial vasoconstriction in PPHN lambs. ecSOD activity was decreased in pulmonary arterial smooth muscle cells (PASMCs) isolated from PPHN lambs relative to controls. Exposure to 95% O 2 to mimic hyperoxic ventilation reduced ecSOD activity in control PASMCs. In both cases, these events were associated with increased protein thiol oxidation, as detected by the redox sensor roGFP. Accordingly, exogenous H 2O 2 decreased ecSOD activity in control PASMCs, and PEG-catalase restored ecSOD activity in PPHN PASMCs. In intact animal studies, ecSOD activity was decreased in fetal PPHN lambs, and in PPHN lambs ventilated with 100% O 2 relative to controls. In ventilated PPHN lambs, administration of a single dose of intratracheal PEG-catalase enhanced ecSOD activity, reduced superoxide levels, and improved oxygenation. We propose that H 2O 2 generated by PPHN and hyperoxia inactivates ecSOD, and intratracheal catalase enhances enzyme function. The associated decrease in extracellular superoxide augments vasodilation, suggesting that H 2O 2 scavengers may represent an effective therapy in the clinical management of PPHN.

Original languageEnglish (US)
Pages (from-to)1497-1506
Number of pages10
JournalAntioxidants and Redox Signaling
Issue number6
StatePublished - Sep 15 2011
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology


Dive into the research topics of 'Hydrogen peroxide regulates extracellular superoxide dismutase activity and expression in neonatal pulmonary hypertension'. Together they form a unique fingerprint.

Cite this