@inproceedings{601d03fa5a83456b888457dd6cc47313,
title = "Imaging a seizure model in zebrafish with structured illumination light sheet microscopy",
abstract = "Zebrafish are a promising vertebrate model for elucidating how neural circuits generate behavior under normal and pathological conditions. The Baraban group first demonstrated that zebrafish larvae are valuable for investigating seizure events and can be used as a model for epilepsy in humans. Because of their small size and transparency, zebrafish embryos are ideal for imaging seizure activity using calcium indicators. Light-sheet microscopy is well suited to capturing neural activity in zebrafish because it is capable of optical sectioning, high frame rates, and low excitation intensities. We describe work in our lab to use light-sheet microscopy for high-speed long-time imaging of neural activity in wildtype and mutant zebrafish to better understand the connectivity and activity of inhibitory neural networks when GABAergic signaling is altered in vivo. We show that, with light-sheet microscopy, neural activity can be recorded at 23 frames per second in twocolors for over 10 minutes allowing us to capture rare seizure events in mutants. We have further implemented structured illumination to increase resolution and contrast in the vertical and axial directions during high-speed imaging at an effective frame rate of over 7 frames per second.",
keywords = "fluorescence microscopy, light sheet microscopy, neurophotonics, structured illumination microscopy",
author = "Yang Liu and Savannah Dale and Rebecca Ball and Vanleuven, {Ariel J.} and Scott Baraban and Andrew Sornborger and Lauderdale, {James D.} and Peter Kner",
note = "Publisher Copyright: {\textcopyright} COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.; Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV 2018 ; Conference date: 29-01-2018 Through 31-01-2018",
year = "2018",
doi = "10.1117/12.2288326",
language = "English (US)",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Tony Wilson and Cogswell, {Carol J.} and Brown, {Thomas G.}",
booktitle = "Three-Dimensional and Multidimensional Microscopy",
address = "United States",
}