Improving performance of nanoscale ultrasound contrast agents using N,N-diethylacrylamide stabilization

Reshani H. Perera, Hanping Wu, Pubudu Peiris, Christopher Hernandez, Alan Burke, Helen Zhang, Agata A. Exner

Research output: Contribution to journalArticlepeer-review

64 Scopus citations


The design of nanoscale yet highly echogenic agents for imaging outside of the vasculature and for ultrasound-mediated drug delivery remains a formidable challenge. We have previously reported on formulation of echogenic perfluoropropane gas nanobubbles stabilized by a lipid-pluronic surfactant shell. In the current work we describe the development of a new generation of these nanoparticles which consist of perfluoropropane gas stabilized by a surfactant and lipid membrane and a crosslinked network of N,N-diethylacrylamide. The resulting crosslinked nanobubbles (CL-PEG-NB) were 95.2 ± 25.2 nm in diameter and showed significant improvement in stability and retention of echogenic signal over 24 h. In vivo analysis via ultrasound and fluorescence mediated tomography showed greater tumor extravasation and accumulation with CL-PEG-NB compared to microbubbles. Together these results demonstrate the capabilities and advantages of a new, more stable, nanometer-scale ultrasound contrast agent that can be utilized in future work for diagnostic scans and molecular imaging.

Original languageEnglish (US)
Pages (from-to)59-67
Number of pages9
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Issue number1
StatePublished - Jan 1 2017
Externally publishedYes


  • Cancer
  • Extravasation
  • Microbubble
  • Nanobubble
  • Ultrasound contrast agent

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science


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