Influence of MDPB-containing primer on streptococcus mutans biofilm formation in simulated class I restorations

Eugenio Brambilla, Andrei Ionescu, Luigi Fadini, Annalisa Mazzoni, Satoshi Imazato, David Pashley, Lorenzo Breschi, Massimo Gagliani

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Purpose: To evaluate the activity of a methacryloyloxydodecylpyridinium bromide (MDPB)-containing self-etching primer (Clearfil Protect Bond) against Streptococcus mutans and its ability to reduce biofilm formation on standardized experimental Class I restorations in vitro. Materials and Methods: Forty experimental Class I round restorations were prepared on enamel-dentin slabs using different adhesive strategies: group 1 = MDPB-containing adhesive system (Clearfil Protect Bond); group 2 = MDPB-free self-etching adhesive system (Clearfil SE Bond); group 3: MDPB-containing self-etching primer in combination with a fluoride-free bonding agent; group 4: MDPB-free self-etching primer in combination with a fluoride-containing bonding agent; group 5: a three-step etch-and-rinse adhesive system (Adper Scotchbond Multi Purpose). A Streptococcus mutans biofilm was grown for 48 h on the restoration surfaces and subsequently evaluated using scanning electron microscopy on three different areas: enamel, composite, and interface surfaces. Statistical analysis was performed by multiple ANOVA after data transformation. Results: Specimens in groups 2, 4 and 5 showed greater biofilm formation than those in groups 1 and 3 (p < 0.001) on all investigated substrates (enamel, composite, and interface areas). Conclusions: Specimens prepared with an MDPB-containing primer exhibited significant decreases in biofilm formation on Class I restorations in vitro. Further in vitro and in vivo studies are required to clarify the role of quaternary ammonium compounds in reducing bacterial biofilm formation on restoration surfaces.

Original languageEnglish (US)
Pages (from-to)431-438
Number of pages8
JournalJournal of Adhesive Dentistry
Issue number5
StatePublished - 2013


  • Antibacterial agents
  • Biofilms
  • Dentin bonding agents
  • MDPB monomer
  • Streptococcus mutans

ASJC Scopus subject areas

  • Oral Surgery
  • Periodontics
  • Orthodontics


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