TY - JOUR
T1 - Inherent physical characteristics and gene expression differences between alveolar and basal bones
AU - Zakhary, Ibrahim
AU - Alotibi, Fawwaz
AU - Lewis, Jill
AU - Elsalanty, Mohammed
AU - Wenger, Karl
AU - Sharawy, Mohamed
AU - Messer, Regina L.W.
N1 - Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/7/1
Y1 - 2016/7/1
N2 - Objectives The objective of this study was to evaluate the baseline differences between alveolar and basal areas of the rat mandible. Study Design Rat mandibular alveolar and basal bones were evaluated using histology and micro-computed tomography to compare osteocyte number as well as bone density and architecture and polymerase chain reaction to measure gene expression levels. Results Micro-computed tomography data indicated that basal bone is denser and less porous than alveolar bone. Histologic analysis showed that alveolar bone has more osteocytes per unit area compared with basal bone. Real-time polymerase chain reaction results showed higher levels of expression of the following genes in basal bone than in alveolar bone: SOST, E-11, DMP-1, and MEPE. Conclusions Three of these gene products are associated with mature osteocytes, and this suggests that basal bone has more mature osteocyte phenotypes compared with alveolar bone. These findings are suggestive of fewer bone mineralization units and therefore a slower remodeling rate.
AB - Objectives The objective of this study was to evaluate the baseline differences between alveolar and basal areas of the rat mandible. Study Design Rat mandibular alveolar and basal bones were evaluated using histology and micro-computed tomography to compare osteocyte number as well as bone density and architecture and polymerase chain reaction to measure gene expression levels. Results Micro-computed tomography data indicated that basal bone is denser and less porous than alveolar bone. Histologic analysis showed that alveolar bone has more osteocytes per unit area compared with basal bone. Real-time polymerase chain reaction results showed higher levels of expression of the following genes in basal bone than in alveolar bone: SOST, E-11, DMP-1, and MEPE. Conclusions Three of these gene products are associated with mature osteocytes, and this suggests that basal bone has more mature osteocyte phenotypes compared with alveolar bone. These findings are suggestive of fewer bone mineralization units and therefore a slower remodeling rate.
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U2 - 10.1016/j.oooo.2016.01.008
DO - 10.1016/j.oooo.2016.01.008
M3 - Article
C2 - 27039006
AN - SCOPUS:84962124853
SN - 2212-4403
VL - 122
SP - 35
EP - 42
JO - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
JF - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
IS - 1
ER -