Instability of miRNA and cDNAs derivatives in RNA preparations

Valia Bravo; Samuel Rosero; Camillo Ricordi; Ricardo L. Pastori

doi:10.1016/j.bbrc.2006.12.135

Instability of miRNA and cDNAs derivatives in RNA preparations

Valia Bravo, Samuel Rosero, Camillo Ricordi, Ricardo L. Pastori

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

Micro RNAs (miRNAs) are small RNA molecules, which function as important regulators of gene expression. We found that RNA preparation methods commonly utilized for miRNA expression studies yield highly unstable miRNAs. We studied the stability of four miRNAs belonging to different miRNAs families. A significant degradation of these molecules may be observed already three days after RNA isolation. Moreover, the respective cDNAs are highly unstable as well. Our findings indicate that instability of miRNAs and their cDNAs should be considered when designing miRNA expression studies.

Original language	English (US)
Pages (from-to)	1052-1055
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	353
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2006.12.135
State	Published - Feb 23 2007
Externally published	Yes

Keywords

MiRNA cDNA stability
MicroRNA isolation
MicroRNA stability
Quantification of microRNAs

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2006.12.135

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title = "Instability of miRNA and cDNAs derivatives in RNA preparations",

abstract = "Micro RNAs (miRNAs) are small RNA molecules, which function as important regulators of gene expression. We found that RNA preparation methods commonly utilized for miRNA expression studies yield highly unstable miRNAs. We studied the stability of four miRNAs belonging to different miRNAs families. A significant degradation of these molecules may be observed already three days after RNA isolation. Moreover, the respective cDNAs are highly unstable as well. Our findings indicate that instability of miRNAs and their cDNAs should be considered when designing miRNA expression studies.",

keywords = "MiRNA cDNA stability, MicroRNA isolation, MicroRNA stability, Quantification of microRNAs",

author = "Valia Bravo and Samuel Rosero and Camillo Ricordi and Pastori, {Ricardo L.}",

note = "Funding Information: This work was supported by a grant from the American Diabetes Association (R.L.P.), the Diabetes Research Institute Foundation, the Peacock foundation and Foundation for Diabetes Research, New Jersey. We thank Drs. Dagmar Klein, Alberto Pugliese, and Juan Dominguez-Bendala for critical reading of the manuscript. We thank the University of Miami DNA microarray Core Facility ( http://www.biomed.miami.edu/arrays ) for technical assistance with DNA electrophoresis and quantification using the 2100 Bioanalizer, (Agilent). Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

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doi = "10.1016/j.bbrc.2006.12.135",

language = "English (US)",

volume = "353",

pages = "1052--1055",

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TY - JOUR

T1 - Instability of miRNA and cDNAs derivatives in RNA preparations

AU - Bravo, Valia

AU - Rosero, Samuel

AU - Ricordi, Camillo

AU - Pastori, Ricardo L.

N1 - Funding Information: This work was supported by a grant from the American Diabetes Association (R.L.P.), the Diabetes Research Institute Foundation, the Peacock foundation and Foundation for Diabetes Research, New Jersey. We thank Drs. Dagmar Klein, Alberto Pugliese, and Juan Dominguez-Bendala for critical reading of the manuscript. We thank the University of Miami DNA microarray Core Facility ( http://www.biomed.miami.edu/arrays ) for technical assistance with DNA electrophoresis and quantification using the 2100 Bioanalizer, (Agilent). Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2007/2/23

Y1 - 2007/2/23

N2 - Micro RNAs (miRNAs) are small RNA molecules, which function as important regulators of gene expression. We found that RNA preparation methods commonly utilized for miRNA expression studies yield highly unstable miRNAs. We studied the stability of four miRNAs belonging to different miRNAs families. A significant degradation of these molecules may be observed already three days after RNA isolation. Moreover, the respective cDNAs are highly unstable as well. Our findings indicate that instability of miRNAs and their cDNAs should be considered when designing miRNA expression studies.

AB - Micro RNAs (miRNAs) are small RNA molecules, which function as important regulators of gene expression. We found that RNA preparation methods commonly utilized for miRNA expression studies yield highly unstable miRNAs. We studied the stability of four miRNAs belonging to different miRNAs families. A significant degradation of these molecules may be observed already three days after RNA isolation. Moreover, the respective cDNAs are highly unstable as well. Our findings indicate that instability of miRNAs and their cDNAs should be considered when designing miRNA expression studies.

KW - MiRNA cDNA stability

KW - MicroRNA isolation

KW - MicroRNA stability

KW - Quantification of microRNAs

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DO - 10.1016/j.bbrc.2006.12.135

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Instability of miRNA and cDNAs derivatives in RNA preparations

Abstract

Keywords

ASJC Scopus subject areas

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