Using a specific antiserum recognizing recombinant rat interleukin-1β (IL-1β), immunoreactive material was localized to cytoplasmic granules in anterior pituitary endocrine cells and colocalized with TSH in thyrotropes. Authenticity was established by Northern blot hybridization using a specific rat IL-1β cRNA probe, revealing a 1.8-kilobase mRNA identical to that in the spleen. The marked increase in anterior pituitary IL-1β message after the administration of bacterial lipopolysaccharide, raises the possibility that IL-1β may be involved in paracrine or autocrine regulation of pituitary function during infectious challenge.
|Original language||English (US)|
|Number of pages||6|
|State||Published - Jun 1990|
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