Involvement of endothelial Man and Gal-binding lectins in sensing the flow in coronary arteries

Alma Barajas-Espinosa, Juan Ramiro-Diaz, Erika Briones-Cerecero, Erika Chi-Ahumada, Ana Barba De La Rosa, Blanca Arroyo-Flores, Rafael Rubio

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The coronary endothelial luminal membrane (CELM) glycocalyx has diverse molecules involved in blood flow signal transduction. Evidence suggests that some of these structures may be lectinic. To test this, we synthesized two monosaccharide polymers (Mon-Pols) made of Mannose (Man-Pol) or Galactose (Gal-Pol) covalently coupled to Dextran (70kDa) and used them as lectin affinity probes. In situ intracoronary infusion of both polymers resulted in CELM-binding but only Man-Pol caused a reduction in flow-induced positive inotropism and dromotropism. To demonstrate that our lectinic probes could bind to CELM lectins, a representative CELM protein fraction was isolated via intracoronary infusion of a cationic silica colloid and either Mannose- or Galactose-binding lectins were purified from the CELM protein fraction using the corresponding Mon-Pol affinity chromatography resin. Resinbound CELM proteins were eluted with the corresponding monosaccharide. 2D-SDS-PAGE (pH 4-7) revealed 9 Mannose- and approximately 100 Galactose-selective CELM lectins. In summary, the CELM glycocalyx contains Mannose- and Galactose-binding lectins that may be involved in translating coronary flow into a cardiac parenchymal response.

Original languageEnglish (US)
Pages (from-to)5421-5431
Number of pages11
JournalFrontiers in Bioscience
Issue number14
StatePublished - May 1 2008
Externally publishedYes


  • Coronary
  • Endothelium
  • Flow sensation
  • Lectin
  • Monosaccharide

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)


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