Involvement of headless myosin X in the motility of immortalized gonadotropin-releasing hormone neuronal cells

Jun Jie Wang; Xiu Qing Fu; Yu Guang Guo; Lin Yuan; Qian Qian Gao; Hua Li Yu; Heng Liang Shi; Xing Zhi Wang; Wen Cheng Xiong; Xiao Juan Zhu

doi:10.1016/j.cellbi.2009.02.006

Involvement of headless myosin X in the motility of immortalized gonadotropin-releasing hormone neuronal cells

Jun Jie Wang, Xiu Qing Fu, Yu Guang Guo, Lin Yuan, Qian Qian Gao, Hua Li Yu, Heng Liang Shi, Xing Zhi Wang, Wen Cheng Xiong, Xiao Juan Zhu

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Myosin X (Myo X), an unconventional myosin with a tail homology 4-band 4.1/ezrin/radixin/moesin (MyTH4-FERM) tail, is expressed ubiquitously in various mammalian tissues. In addition to the full-length Myo X (Myo X FL), a headless form is synthesized in the brain. So far, little is known about the function of this motor-less Myo X. In this study, the role of the headless Myo X was investigated in immortalized gonadotropin-releasing hormone (GnRH) neuronal cells, NLT. NLT cells overexpressing the headless Myo X formed fewer focal adhesions and spread more slowly than the wild-type NLT cells and GFP-expressing NLT cells. In chemomigration assays, the NLT cells overexpressing the headless Myo X migrated shorter distances and had fewer migratory cells compared with the control NLT cells.

Original language	English (US)
Pages (from-to)	578-585
Number of pages	8
Journal	Cell Biology International
Volume	33
Issue number	5
DOIs	https://doi.org/10.1016/j.cellbi.2009.02.006
State	Published - May 2009
Externally published	Yes

Keywords

Adhesion
Filopodia
Migration
Overexpression

ASJC Scopus subject areas

Cell Biology

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10.1016/j.cellbi.2009.02.006

Cite this

@article{ade85c3452a644b9bbd18dee9ae9934a,

title = "Involvement of headless myosin X in the motility of immortalized gonadotropin-releasing hormone neuronal cells",

abstract = "Myosin X (Myo X), an unconventional myosin with a tail homology 4-band 4.1/ezrin/radixin/moesin (MyTH4-FERM) tail, is expressed ubiquitously in various mammalian tissues. In addition to the full-length Myo X (Myo X FL), a headless form is synthesized in the brain. So far, little is known about the function of this motor-less Myo X. In this study, the role of the headless Myo X was investigated in immortalized gonadotropin-releasing hormone (GnRH) neuronal cells, NLT. NLT cells overexpressing the headless Myo X formed fewer focal adhesions and spread more slowly than the wild-type NLT cells and GFP-expressing NLT cells. In chemomigration assays, the NLT cells overexpressing the headless Myo X migrated shorter distances and had fewer migratory cells compared with the control NLT cells.",

keywords = "Adhesion, Filopodia, Migration, Overexpression",

author = "Wang, {Jun Jie} and Fu, {Xiu Qing} and Guo, {Yu Guang} and Lin Yuan and Gao, {Qian Qian} and Yu, {Hua Li} and Shi, {Heng Liang} and Wang, {Xing Zhi} and Xiong, {Wen Cheng} and Zhu, {Xiao Juan}",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (30670689) and the Program for New Century Excellent Talents in University (NCET-07-0173), Specialized Research Fund for the Doctoral Program of High Education (20060200008) and Scientific Research Foundation for the Returned Overseas Chinese Scholars from the Ministry of Education of China.",

year = "2009",

month = may,

doi = "10.1016/j.cellbi.2009.02.006",

language = "English (US)",

volume = "33",

pages = "578--585",

journal = "Cell Biology International",

issn = "1065-6995",

publisher = "Portland Press Ltd.",

number = "5",

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TY - JOUR

T1 - Involvement of headless myosin X in the motility of immortalized gonadotropin-releasing hormone neuronal cells

AU - Wang, Jun Jie

AU - Fu, Xiu Qing

AU - Guo, Yu Guang

AU - Yuan, Lin

AU - Gao, Qian Qian

AU - Yu, Hua Li

AU - Shi, Heng Liang

AU - Wang, Xing Zhi

AU - Xiong, Wen Cheng

AU - Zhu, Xiao Juan

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (30670689) and the Program for New Century Excellent Talents in University (NCET-07-0173), Specialized Research Fund for the Doctoral Program of High Education (20060200008) and Scientific Research Foundation for the Returned Overseas Chinese Scholars from the Ministry of Education of China.

PY - 2009/5

Y1 - 2009/5

N2 - Myosin X (Myo X), an unconventional myosin with a tail homology 4-band 4.1/ezrin/radixin/moesin (MyTH4-FERM) tail, is expressed ubiquitously in various mammalian tissues. In addition to the full-length Myo X (Myo X FL), a headless form is synthesized in the brain. So far, little is known about the function of this motor-less Myo X. In this study, the role of the headless Myo X was investigated in immortalized gonadotropin-releasing hormone (GnRH) neuronal cells, NLT. NLT cells overexpressing the headless Myo X formed fewer focal adhesions and spread more slowly than the wild-type NLT cells and GFP-expressing NLT cells. In chemomigration assays, the NLT cells overexpressing the headless Myo X migrated shorter distances and had fewer migratory cells compared with the control NLT cells.

AB - Myosin X (Myo X), an unconventional myosin with a tail homology 4-band 4.1/ezrin/radixin/moesin (MyTH4-FERM) tail, is expressed ubiquitously in various mammalian tissues. In addition to the full-length Myo X (Myo X FL), a headless form is synthesized in the brain. So far, little is known about the function of this motor-less Myo X. In this study, the role of the headless Myo X was investigated in immortalized gonadotropin-releasing hormone (GnRH) neuronal cells, NLT. NLT cells overexpressing the headless Myo X formed fewer focal adhesions and spread more slowly than the wild-type NLT cells and GFP-expressing NLT cells. In chemomigration assays, the NLT cells overexpressing the headless Myo X migrated shorter distances and had fewer migratory cells compared with the control NLT cells.

KW - Adhesion

KW - Filopodia

KW - Migration

KW - Overexpression

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Involvement of headless myosin X in the motility of immortalized gonadotropin-releasing hormone neuronal cells

Abstract

Keywords

ASJC Scopus subject areas

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