Iron attenuates nitric oxide level and iNOS expression in endotoxin-treated mice

Andrei M. Komarov; David L. Mattson; I. Tong Mak; William B. Weglicki

doi:10.1016/S0014-5793(98)00181-1

Iron attenuates nitric oxide level and iNOS expression in endotoxin-treated mice

Andrei M. Komarov, David L. Mattson, I. Tong Mak, William B. Weglicki

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22 Scopus citations

Abstract

The effect of exogenous Fe-citrate complex (Fe doses of 120 and 230 μmol/kg) on nitric oxide (NO) production in vivo has been studied in blood and liver tissue of endotoxin-treated mice. Fe-citrate complex was administered to mice subcutaneously at the same time with intravenous injection of Escherichia coli lipopolysaccharide (LPS). Iron-dependent decrease in NO₂^-/NO₃^- and nitrosyl hemoglobin levels in blood of animals was detected at 6 h after LPS administration, suggesting systemic attenuation of NO generation. NO production in the liver tissue of LPS-treated mice was decreased after Fe administration judging from the amount of mononitrosyl-iron complexes formed in the tissue by diethyldithiocarbamate. The iNOS protein determination in the liver tissue of LPS-treated mice demonstrated iron-dependent inhibition of iNOS expression. We have found previously that exogenous iron does not affect systemic NO level when it is given at 6 h after LPS injection, i.e. after iNOS expression. This is a first report demonstrating iron-dependent iNOS down-regulation in endotoxin-treated mice.

Original language	English (US)
Pages (from-to)	253-256
Number of pages	4
Journal	FEBS Letters
Volume	424
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(98)00181-1
State	Published - Mar 13 1998
Externally published	Yes

Keywords

Inducible nitric oxide synthase
Iron
Nitric oxide
Nitrosyl iron complex
Septic shock
Spin trapping

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(98)00181-1

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title = "Iron attenuates nitric oxide level and iNOS expression in endotoxin-treated mice",

abstract = "The effect of exogenous Fe-citrate complex (Fe doses of 120 and 230 μmol/kg) on nitric oxide (NO) production in vivo has been studied in blood and liver tissue of endotoxin-treated mice. Fe-citrate complex was administered to mice subcutaneously at the same time with intravenous injection of Escherichia coli lipopolysaccharide (LPS). Iron-dependent decrease in NO2-/NO3- and nitrosyl hemoglobin levels in blood of animals was detected at 6 h after LPS administration, suggesting systemic attenuation of NO generation. NO production in the liver tissue of LPS-treated mice was decreased after Fe administration judging from the amount of mononitrosyl-iron complexes formed in the tissue by diethyldithiocarbamate. The iNOS protein determination in the liver tissue of LPS-treated mice demonstrated iron-dependent inhibition of iNOS expression. We have found previously that exogenous iron does not affect systemic NO level when it is given at 6 h after LPS injection, i.e. after iNOS expression. This is a first report demonstrating iron-dependent iNOS down-regulation in endotoxin-treated mice.",

keywords = "Inducible nitric oxide synthase, Iron, Nitric oxide, Nitrosyl iron complex, Septic shock, Spin trapping",

author = "Komarov, {Andrei M.} and Mattson, {David L.} and Mak, {I. Tong} and Weglicki, {William B.}",

note = "Funding Information: This work was supported in part by NIH Grant RO1-HL36418.",

year = "1998",

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doi = "10.1016/S0014-5793(98)00181-1",

language = "English (US)",

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T1 - Iron attenuates nitric oxide level and iNOS expression in endotoxin-treated mice

AU - Komarov, Andrei M.

AU - Mattson, David L.

AU - Mak, I. Tong

AU - Weglicki, William B.

N1 - Funding Information: This work was supported in part by NIH Grant RO1-HL36418.

PY - 1998/3/13

Y1 - 1998/3/13

N2 - The effect of exogenous Fe-citrate complex (Fe doses of 120 and 230 μmol/kg) on nitric oxide (NO) production in vivo has been studied in blood and liver tissue of endotoxin-treated mice. Fe-citrate complex was administered to mice subcutaneously at the same time with intravenous injection of Escherichia coli lipopolysaccharide (LPS). Iron-dependent decrease in NO2-/NO3- and nitrosyl hemoglobin levels in blood of animals was detected at 6 h after LPS administration, suggesting systemic attenuation of NO generation. NO production in the liver tissue of LPS-treated mice was decreased after Fe administration judging from the amount of mononitrosyl-iron complexes formed in the tissue by diethyldithiocarbamate. The iNOS protein determination in the liver tissue of LPS-treated mice demonstrated iron-dependent inhibition of iNOS expression. We have found previously that exogenous iron does not affect systemic NO level when it is given at 6 h after LPS injection, i.e. after iNOS expression. This is a first report demonstrating iron-dependent iNOS down-regulation in endotoxin-treated mice.

AB - The effect of exogenous Fe-citrate complex (Fe doses of 120 and 230 μmol/kg) on nitric oxide (NO) production in vivo has been studied in blood and liver tissue of endotoxin-treated mice. Fe-citrate complex was administered to mice subcutaneously at the same time with intravenous injection of Escherichia coli lipopolysaccharide (LPS). Iron-dependent decrease in NO2-/NO3- and nitrosyl hemoglobin levels in blood of animals was detected at 6 h after LPS administration, suggesting systemic attenuation of NO generation. NO production in the liver tissue of LPS-treated mice was decreased after Fe administration judging from the amount of mononitrosyl-iron complexes formed in the tissue by diethyldithiocarbamate. The iNOS protein determination in the liver tissue of LPS-treated mice demonstrated iron-dependent inhibition of iNOS expression. We have found previously that exogenous iron does not affect systemic NO level when it is given at 6 h after LPS injection, i.e. after iNOS expression. This is a first report demonstrating iron-dependent iNOS down-regulation in endotoxin-treated mice.

KW - Inducible nitric oxide synthase

KW - Iron

KW - Nitric oxide

KW - Nitrosyl iron complex

KW - Septic shock

KW - Spin trapping

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Iron attenuates nitric oxide level and iNOS expression in endotoxin-treated mice

Abstract

Keywords

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