Abstract
Sequence data suggest that Japanese encephalitis virus (JEV) protein NS3 is a multifunctional protein with sequence motifs characteristic of a protease and a helicase. To examine the functions of JEV-NS3, a fusion protein of NS3 in Escherichia coli was generated. Analysis by Western blot using monospecific rabbit antisera generated against the fusion protein (anti-MBJEN3) showed that NS3 was localized in the membrane fraction of JEV-infected cells and the particulate fraction of bacteria extracts. The addition of anti-MBJEN3 sera reduced JEV-specific RNA synthesis activity in a in vitro system. In addition, NS3 was shown to exhibit RNA binding and ATPase activities, suggesting this protein has an important role in viral RNA replication in virus-infected cells.
Original language | English (US) |
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Pages (from-to) | 105-112 |
Number of pages | 8 |
Journal | Virus Genes |
Volume | 9 |
Issue number | 2 |
DOIs | |
State | Published - Jan 1 1995 |
Externally published | Yes |
Keywords
- ATPase
- Japanese encephalitis virus
- RNA binding
- expressed protein
- nonstructural protein NS3
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Virology