TY - JOUR
T1 - lacZ-neoR transfected glioma cells in syngeneic rats
T2 - Growth pattern and characterization of the host immune response against cells transplanted inside and outside the CNS
AU - Visted, Therese
AU - Thorsen, Jon
AU - Thorsen, Frits
AU - Read, Tracy Ann
AU - Ulvestad, Elling
AU - Engebraaten, Olav
AU - SØrensen, Dag
AU - Ylä-Herttuala, Seppo
AU - Tyynela, Kristina
AU - Rucklidge, Garry
AU - Edvardsen, Klaus
AU - Bjerkvig, Rolf
AU - Lund-Johansen, Morten
PY - 2000/1/15
Y1 - 2000/1/15
N2 - The rat glioma cell lines BT4C and BT4Cn were stably transfected with the bacterial lacZ-neomycin resistance (neoR) gene construct. Both transfected (BT4ClacZ and BT4CnlacZ) and untransfected cell lines were injected intracerebrally and subcutaneously into rats. Survival time, morphology, growth rate and immunological properties of the tumors were studied. Survival time was significantly prolonged after intracerebral implantation of the transfected cell lines. No similar response was found in nude rats, indicating an immunological response towards the lacZ-neoR- transfected cells in immunocompetent animals. Morphological observations showed that the lacZ-neoR-transfected gliomas were smaller and had a distinct boundary with the normal brain tissue, whereas the parental cell lines revealed a more diffuse growth pattern. Immunostaining showed a higher proportion of immunocompetent cells infiltrating the lacZ-neoR-transfected tumors. After s.c. injection, the lacZ-neoR-transfected BT4C cell line had a prolonged lag phase before assuming a growth rate similar to that of the parental cells. The BT4CnvlacZ tumors initially grew fastest, but then disappeared within 3 weeks. A similar response was observed with mock- transfected tumor cells. A 3HTdR-incorporation assay on spleen cells from rats transplanted s.c. with BT4CnvlacZ cells showed a 10-fold increase in cell activation as compared with rats with BT4Cn tumors. A humoral response towards the transfected cells was verified by Western-blot analyses.
AB - The rat glioma cell lines BT4C and BT4Cn were stably transfected with the bacterial lacZ-neomycin resistance (neoR) gene construct. Both transfected (BT4ClacZ and BT4CnlacZ) and untransfected cell lines were injected intracerebrally and subcutaneously into rats. Survival time, morphology, growth rate and immunological properties of the tumors were studied. Survival time was significantly prolonged after intracerebral implantation of the transfected cell lines. No similar response was found in nude rats, indicating an immunological response towards the lacZ-neoR- transfected cells in immunocompetent animals. Morphological observations showed that the lacZ-neoR-transfected gliomas were smaller and had a distinct boundary with the normal brain tissue, whereas the parental cell lines revealed a more diffuse growth pattern. Immunostaining showed a higher proportion of immunocompetent cells infiltrating the lacZ-neoR-transfected tumors. After s.c. injection, the lacZ-neoR-transfected BT4C cell line had a prolonged lag phase before assuming a growth rate similar to that of the parental cells. The BT4CnvlacZ tumors initially grew fastest, but then disappeared within 3 weeks. A similar response was observed with mock- transfected tumor cells. A 3HTdR-incorporation assay on spleen cells from rats transplanted s.c. with BT4CnvlacZ cells showed a 10-fold increase in cell activation as compared with rats with BT4Cn tumors. A humoral response towards the transfected cells was verified by Western-blot analyses.
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U2 - 10.1002/(SICI)1097-0215(20000115)85:2%3C228::AID-IJC13%3E3.0.CO;2-9
DO - 10.1002/(SICI)1097-0215(20000115)85:2%3C228::AID-IJC13%3E3.0.CO;2-9
M3 - Article
C2 - 10629082
AN - SCOPUS:0034650927
SN - 0020-7136
VL - 85
SP - 228
EP - 235
JO - International Journal of Cancer
JF - International Journal of Cancer
IS - 2
ER -
