Leptin-induced changes in body composition in high fat-fed mice

Ruth B.S. Harris, Tiffany D. Mitchell, Sadie Hebert

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Female C57BL/6J mice were adapted to 10% or 45% kcal fat diets for 8 weeks. Continuous intraperitoneal infusion of 10 μg of leptin/day from a miniosmotic pump transiently inhibited food intake in low fat-fed but not high fat-fed mice. In contrast, both low and high fat-fed leptin-infused mice were less fat than their phosphate-buffered saline (PBS) controls after 13 days. Leptin infusion inhibited insulin release but did not change glucose clearance in low fat-fed mice during a glucose tolerance test. A single intraperitoneal injection of 30 μg of leptin inhibited 24-hr energy intake and inhibited weight gain in both low and high fat-fed mice. Insulin responsiveness was improved in high fat-fed mice during an insulin sensitivity test due to an exaggerated elevation of circulating insulin concentrations. Thus, leptin infusion reduced adiposity independently of energy intake in high fat-fed mice and improved insulin sensitivity in low fat-fed mice, whereas leptin injections, which produced much greater, but transient, increases in serum leptin concentration, inhibited energy intake in both low and high fat-fed mice.

Original languageEnglish (US)
Pages (from-to)24-32
Number of pages9
JournalExperimental Biology and Medicine
Issue number1
StatePublished - Jan 2003
Externally publishedYes


  • Body composition
  • IRS protein
  • Leptin infusion
  • Leptin injection
  • Leptin resistance

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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