Lipopolysaccharide Pretreatment Prevents Medullary Vascular Congestion following Renal Ischemia by Limiting Early Reperfusion of the Medullary Circulation

Sarah R. McLarnon, Katie Wilson, Bansari Patel, Jingping Sun, Christina L. Sartain, Christopher D. Mejias, Jacqueline B. Musall, Jennifer C. Sullivan, Qingqing Wei, Jian Kang Chen, Kelly A. Hyndman, Brendan Marshall, Haichun Yang, Agnes B. Fogo, Paul M. O’Connor

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Background Vascular congestion of the renal medulla—trapped red blood cells in the medullary microvasculature—is a hallmark finding at autopsy in patients with ischemic acute tubular necrosis. Despite this, the pathogenesis of vascular congestion is not well defined. Methods In this study, to investigate the pathogenesis of vascular congestion and its role in promoting renal injury, we assessed renal vascular congestion and tubular injury after ischemia reperfusion in rats pretreated with low-dose LPS or saline (control). We used laser Doppler flowmetry to determine whether pretreatment with low-dose LPS prevented vascular congestion by altering renal hemodynamics during reperfusion. Results We found that vascular congestion originated during the ischemic period in the renal venous circulation. In control animals, the return of blood flow was followed by the development of congestion in the capillary plexus of the outer medulla and severe tubular injury early in reperfusion. Laser Doppler flowmetry indicated that blood flow returned rapidly to the medulla, several minutes before recovery of full cortical perfusion. In contrast, LPS pretreatment prevented both the formation of medullary congestion and its associated tubular injury. Laser Doppler flowmetry in LPS-pretreated rats suggested that limiting early reperfusion of the medulla facilitated this protective effect, because it allowed cortical perfusion to recover and clear congestion from the large cortical veins, which also drain the medulla. Conclusions Blockage of the renal venous vessels and a mismatch in the timing of cortical and medullary reperfusion results in congestion of the outer medulla’s capillary plexus and promotes early tubular injury after renal ischemia. These findings indicate that hemodynamics during reperfusion contribute to the renal medulla’s susceptibility to ischemic injury.

Original languageEnglish (US)
Pages (from-to)769-785
Number of pages17
JournalJournal of the American Society of Nephrology
Issue number4
StatePublished - Apr 2022


  • Acute Kidney Injury/etiology
  • Animals
  • Humans
  • Ischemia/complications
  • Kidney Medulla/blood supply
  • Kidney/pathology
  • Lipopolysaccharides
  • Rats
  • Renal Circulation/physiology
  • Reperfusion Injury/complications
  • Reperfusion/adverse effects


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