TY - JOUR
T1 - Lost in transgenesis
T2 - A User's guide for genetically manipulating the mouse in cardiac research
AU - Davis, Jennifer
AU - Maillet, Marjorie
AU - Miano, Joseph M.
AU - Molkentin, Jeffery D.
PY - 2012/8/31
Y1 - 2012/8/31
N2 - The advent of modern mouse genetics has benefited many fields of diseased-based research over the past 20 years, none perhaps more profoundly than cardiac biology. Indeed, the heart is now arguably one of the easiest tissues to genetically manipulate, given the availability of an ever-growing tool chest of molecular reagents/promoters and "facilitator" mouse lines. It is now possible to modify the expression of essentially any gene or partial gene product in the mouse heart at any time, either gain or loss of function. This review is designed as a handbook for the nonmouse geneticist and/or junior investigator to permit the successful manipulation of any gene or RNA product in the heart, while avoiding artifacts. In the present review, guidelines, pitfalls, and limitations are presented so that rigorous and appropriate examination of cardiac genotype-phenotype relationships can be performed. This review uses examples from the field to illustrate the vast spectrum of experimental and design details that must be considered when using genetically modified mouse models to study cardiac biology.
AB - The advent of modern mouse genetics has benefited many fields of diseased-based research over the past 20 years, none perhaps more profoundly than cardiac biology. Indeed, the heart is now arguably one of the easiest tissues to genetically manipulate, given the availability of an ever-growing tool chest of molecular reagents/promoters and "facilitator" mouse lines. It is now possible to modify the expression of essentially any gene or partial gene product in the mouse heart at any time, either gain or loss of function. This review is designed as a handbook for the nonmouse geneticist and/or junior investigator to permit the successful manipulation of any gene or RNA product in the heart, while avoiding artifacts. In the present review, guidelines, pitfalls, and limitations are presented so that rigorous and appropriate examination of cardiac genotype-phenotype relationships can be performed. This review uses examples from the field to illustrate the vast spectrum of experimental and design details that must be considered when using genetically modified mouse models to study cardiac biology.
KW - genetics
KW - mouse heart development
KW - transgene
KW - transgenic mice
UR - http://www.scopus.com/inward/record.url?scp=84865838098&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84865838098&partnerID=8YFLogxK
U2 - 10.1161/CIRCRESAHA.111.262717
DO - 10.1161/CIRCRESAHA.111.262717
M3 - Review article
C2 - 22935533
AN - SCOPUS:84865838098
SN - 0009-7330
VL - 111
SP - 761
EP - 777
JO - Circulation research
JF - Circulation research
IS - 6
ER -