LTP in cultured hippocampal-entorhinal cortex slices from young adult (P25-30) rats

Jill K. Leutgeb, Julietta U. Frey, Thomas Behnisch

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


Cultured hippocampal neurons and immature organotypic slice cultures overcome temporal limitations of acute hippocampal slices and have been useful for investigating long-lasting plasticity. Difficulties with culturing adult neurons have restricted such studies to preparations from embryonic, perinatal, and juvenile tissue. By improving the methods for culturing and maintaining hippocampal-entorhinal cortex slices obtained from mature rats (P25-30), we show that their use in long-term electrophysiological investigations is feasible. Our cultured slices maintained an intact and functional trisynaptic cascade, normal synaptic function, and reliable long-term recording stability for at least 14 days in vitro. The electrophysiological properties and, in particular, the induction of long-term potentiation (LTP) in our mature organotypic slices were highly sensitive to dissection and tissue culture techniques. We present data describing the extracellular stimulation requirements for LTP-induction and its long-lasting maintenance (>4 h) at the Schaffer-collateral-CA1 synapse, and show that such changes in synaptic efficiency are NMDA receptor dependent. Our hippocampal-entorhinal cortex cultures from mature tissue can retain the electrophysiological properties required for long-term plasticity for several weeks in vitro.

Original languageEnglish (US)
Pages (from-to)19-32
Number of pages14
JournalJournal of Neuroscience Methods
Issue number1
StatePublished - Nov 30 2003
Externally publishedYes


  • Hippocampus
  • LTP
  • Organotypic cultures
  • Synaptic plasticity

ASJC Scopus subject areas

  • Neuroscience(all)


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