Matrix metalloproteinase-2 expression induced by two different adhesive systems on human pulp fibroblasts

Giovanna Orsini, Annalisa Mazzoni, Monia Orciani, Angelo Putignano, Maurizio Procaccini, Mirella Falconi, David H. Pashley, Franklin R. Tay, Lorenzo Breschi

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Introduction: This study evaluated the expression of matrix metalloproteinase-2 (MMP-2) in primary cultures of human pulp fibroblasts (HPFs) when exposed to extracts from dentin-bonding systems. Methods: Polymerized resin disks of the bonding agent of a 2-step self-etch adhesive (TechBond, Isasan, Rovello Porro, Italy) or of the primer/bonding agent a 2-step etch-and-rinse adhesive (Optibond Solo; Sybron-Kerr, Orange, CA) were immersed in HPF culture medium for 24 or 96 hours. HPFs were incubated in the adhesive-conditioned or control (untreated) culture medium for 24 hours. Western blot and immunofluorescence analyses were performed to assay MMP-2 expression. Results: MMP-2 expression levels in HPFs cultured for 24 hours in culture medium were similar in both the control and experimental media groups showing a faint band at 67 kDa. Conversely, the HPFs incubated in the medium that contain polymerized resin disks for 96 hours showed increased MMP-2 expression compared with the untreated medium. The self-etch adhesive displayed the most pronounced induction of MMP-2 expression. These findings were confirmed by immunofluorescence analysis. Conclusions: HPFs display increased MMP-2 expression after 96 hours of conditioning of the HPF culture medium with polymerized disks of dentin bonding systems. This MMP-2 expression/activation may represent a defence mechanism exhibited by HPFs towards monomers eluted from the dentin bonding systems.

Original languageEnglish (US)
Pages (from-to)1663-1667
Number of pages5
JournalJournal of endodontics
Issue number12
StatePublished - Dec 2011


  • Dentin bonding systems
  • fibroblasts
  • matrix metalloproteinase-2

ASJC Scopus subject areas

  • Dentistry(all)


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