Abstract
Insulin and insulin-like growth factor I (IGF-I) influence numerous metabolic and mitogenic processes; these hormones also have vasoactive properties. This study examined mechanisms involved in insulin- and IGF-I-induced dilation in canine conduit and microvascular coronary segments. Tension of coronary artery segments was measured after constriction with PGF(2α). Internal diameter of coronary microvessels (resting diameter = 112.6 ± 10.1 μm) was measured after endothelin constriction. Vessels were incubated in control (Krebs) solution and were treated with N(w)-nitro-L-arginine (L-NA), indomethacin, or K+ channel inhibitors. After constriction, cumulative doses of insulin or IGF-I (0.1-100 ng/ml) were administered. In conduit arteries, insulin produced modest maximal relaxation (32 ± 5%) compared with IGF-I (66 ± 12%). Vasodilation was attenuated by nitric oxide synthase (NOS) and cyclooxygenase inhibition and was blocked with KCl constriction. Coronary microvascular relaxation to insulin and IGF-I was not altered by L-NA, indomethacin, tetraethylammonium chloride, glibenclamide, charybdotoxin, and apamin; however, tetrabutylammonium chloride attenuated the response. In conclusion, insulin and IGF-I cause vasodilation in canine coronary conduit arteries and microvessels. In conduit vessels, NOS/cyclooxygenase pathways are involved in the vasodilation. In microvessels, relaxation to insulin and IGF-I is not mediated by NOS/cyclooxygenase pathways but rather through K+-dependent mechanisms.
Original language | English (US) |
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Pages (from-to) | E176-E181 |
Journal | American Journal of Physiology - Endocrinology and Metabolism |
Volume | 279 |
Issue number | 1 42-1 |
DOIs | |
State | Published - Jul 1 2000 |
Externally published | Yes |
Keywords
- Coronary circulation
- Coronary microcirculation
- Diabetes mellitus
- Dogs
- Potassium channels
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Physiology
- Physiology (medical)