Micromorphology of resin/dentin interfaces using 4th and 5th generation dual-curing adhesive/cement systems: A confocal laser scanning microscope analysis

Cesar A.G. Arrais, Katsuia Miyake, Frederick A. Rueggeberg, David H. Pashley, Marcelo Giannini

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Purpose: This study evaluated the differential composition of resin/dentin interfaces of indirect restorations created by the application of 4th and 5th generation dual-curing luting systems (bonding agents/resin cements), when each material was either light cured or allowed to self-cure. Materials and Methods: Occlusal flat dentin surfaces of 60 human third molars were assigned into 12 groups (n = 5) according to curing mode and dual-curing cementing system: 4th generation All Bond2 (AB2)/Duolink (Bisco) and 5th generation (B1) Bond1/Lute-it (Pentron). Fluorescein-labeled dextran (FDx) was mixed with the bonding agents, while rhodamine-labeled dextran (RhDx) was incorporated into resin cements and Pre-Bond resin from AB2. Resin cements were applied to 2-mm-thick, precured resin composite disks (Z250, 3M ESPE), which were fixed to dentin surfaces containing adhesive resin in either cured (light cured; LC) or uncured (self-cured; SC) states. The restored teeth were light activated (XL3000, 3M ESPE) according to the manufacturers’ instructions (LRC) or allowed to self-cure (SRC), were stored for 24 h, and then vertically, serially sectioned into 1-mm-thick slabs, which were analyzed using confocal laser scanning microscopy. Fluorescent additives indicated where individual components of the bonding/cement systems were located. Additional specimens were prepared and analyzed using a conventional scanning electron microscope. Results: AB2/LC and B1/LC exhibited nonuniform primer/adhesive layer thickness. AB2/SC showed adhesive resin penetration within the primed dentin, and resin cement penetration at the entrance of the dentin tubules. B1/SC/LRC demonstrated resin cement penetration within the hybrid layer and into the dentin tubules. More resin cement penetration was observed in B1/SC/SRC groups than in its LRC equivalent. Conclusion: The morphological features and component interactions among materials at resin/dentin interfaces are related to the activation modes of the primer/adhesive layer and of the resin cement used.

Original languageEnglish (US)
Pages (from-to)15-26
Number of pages12
JournalJournal of Adhesive Dentistry
Issue number1
StatePublished - Jan 1 2009


  • Adhesive interfacial morphology
  • Confocal laser scanning microscopy
  • Dual-curing adhesive systems
  • Dual-curing resin cements
  • Indirect restorations

ASJC Scopus subject areas

  • Orthodontics
  • Oral Surgery
  • Periodontics


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