Modulation of soluble and membrane-bound TNF-induced phenotypic and functional changes of human brain microvascular endothelial cells by recombinant TNF binding protein I

J. Lou; A. Ythier; D. Burger; L. Zheng; P. Juillard; Rudolf Lucas; J. M. Dayer; G. E. Grau

doi:10.1016/S0165-5728(97)00067-2

Modulation of soluble and membrane-bound TNF-induced phenotypic and functional changes of human brain microvascular endothelial cells by recombinant TNF binding protein I

J. Lou, A. Ythier, D. Burger, L. Zheng, P. Juillard, Rudolf Lucas, J. M. Dayer, G. E. Grau

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

In this study, the effects of TNF binding protein I (TBP I) on TNF- induced changes of human brain microvascular endothelial cells (MVEC) were investigated. TBP I completely abolished TNF-induced IL-6 production and E- selectin induction, while it partially inhibited TNF-induced IL-8 production and up-regulation of ICAM-I and VCAM-I. Moreover, TBP I significantly inhibited TNF-induced cytotoxicity and leukocyte adherence on human brain MVEC. The inhibitory activity of TBP I for TNF was dose-dependent and related to the time of administration after TNF stimulation. In addition, TBP I inhibited membrane-bound TNF induced activation of human brain MVEC, but the concentration required was about 10-fold higher than that for soluble TNF. These results indicate a therapeutic potential for TBP I in diseases of the central nervous system associated with TNF overproduction.

Original language	English (US)
Pages (from-to)	107-115
Number of pages	9
Journal	Journal of Neuroimmunology
Volume	77
Issue number	1
DOIs	https://doi.org/10.1016/S0165-5728(97)00067-2
State	Published - Jul 1997
Externally published	Yes

Keywords

Adhesion molecules
Brain microvascular endothelial cells
Cytoadherence
Cytotoxicity
TNF
TNF binding protein I

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0165-5728(97)00067-2

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@article{4de1a7aed49b470384434d1f5d619d43,

title = "Modulation of soluble and membrane-bound TNF-induced phenotypic and functional changes of human brain microvascular endothelial cells by recombinant TNF binding protein I",

abstract = "In this study, the effects of TNF binding protein I (TBP I) on TNF- induced changes of human brain microvascular endothelial cells (MVEC) were investigated. TBP I completely abolished TNF-induced IL-6 production and E- selectin induction, while it partially inhibited TNF-induced IL-8 production and up-regulation of ICAM-I and VCAM-I. Moreover, TBP I significantly inhibited TNF-induced cytotoxicity and leukocyte adherence on human brain MVEC. The inhibitory activity of TBP I for TNF was dose-dependent and related to the time of administration after TNF stimulation. In addition, TBP I inhibited membrane-bound TNF induced activation of human brain MVEC, but the concentration required was about 10-fold higher than that for soluble TNF. These results indicate a therapeutic potential for TBP I in diseases of the central nervous system associated with TNF overproduction.",

keywords = "Adhesion molecules, Brain microvascular endothelial cells, Cytoadherence, Cytotoxicity, TNF, TNF binding protein I",

author = "J. Lou and A. Ythier and D. Burger and L. Zheng and P. Juillard and Rudolf Lucas and Dayer, {J. M.} and Grau, {G. E.}",

note = "Funding Information: This work was supported by grants from Ares-Serono, Geneva, Switzerland (G.E.G.), the Swiss Society for Multiple Sclerosis (to D.B. and G.E.G), the ARSEP, Paris (to G.E.G) and the Swiss National Science Foundation (to G.E.G. and J.-M.D). We thank Dr. D. Wallach (Rehovot), A.J.H. Gearing (Oxford) and D. Degroote (Fleurus) for their generous gift of antibodies and Dr. B. Chappuis (Transfusion Centre, Geneva University Hospital) for blood aliquots of normal human donors.",

year = "1997",

month = jul,

doi = "10.1016/S0165-5728(97)00067-2",

language = "English (US)",

volume = "77",

pages = "107--115",

journal = "Journal of Neuroimmunology",

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publisher = "Elsevier",

number = "1",

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TY - JOUR

T1 - Modulation of soluble and membrane-bound TNF-induced phenotypic and functional changes of human brain microvascular endothelial cells by recombinant TNF binding protein I

AU - Lou, J.

AU - Ythier, A.

AU - Burger, D.

AU - Zheng, L.

AU - Juillard, P.

AU - Lucas, Rudolf

AU - Dayer, J. M.

AU - Grau, G. E.

N1 - Funding Information: This work was supported by grants from Ares-Serono, Geneva, Switzerland (G.E.G.), the Swiss Society for Multiple Sclerosis (to D.B. and G.E.G), the ARSEP, Paris (to G.E.G) and the Swiss National Science Foundation (to G.E.G. and J.-M.D). We thank Dr. D. Wallach (Rehovot), A.J.H. Gearing (Oxford) and D. Degroote (Fleurus) for their generous gift of antibodies and Dr. B. Chappuis (Transfusion Centre, Geneva University Hospital) for blood aliquots of normal human donors.

PY - 1997/7

Y1 - 1997/7

N2 - In this study, the effects of TNF binding protein I (TBP I) on TNF- induced changes of human brain microvascular endothelial cells (MVEC) were investigated. TBP I completely abolished TNF-induced IL-6 production and E- selectin induction, while it partially inhibited TNF-induced IL-8 production and up-regulation of ICAM-I and VCAM-I. Moreover, TBP I significantly inhibited TNF-induced cytotoxicity and leukocyte adherence on human brain MVEC. The inhibitory activity of TBP I for TNF was dose-dependent and related to the time of administration after TNF stimulation. In addition, TBP I inhibited membrane-bound TNF induced activation of human brain MVEC, but the concentration required was about 10-fold higher than that for soluble TNF. These results indicate a therapeutic potential for TBP I in diseases of the central nervous system associated with TNF overproduction.

AB - In this study, the effects of TNF binding protein I (TBP I) on TNF- induced changes of human brain microvascular endothelial cells (MVEC) were investigated. TBP I completely abolished TNF-induced IL-6 production and E- selectin induction, while it partially inhibited TNF-induced IL-8 production and up-regulation of ICAM-I and VCAM-I. Moreover, TBP I significantly inhibited TNF-induced cytotoxicity and leukocyte adherence on human brain MVEC. The inhibitory activity of TBP I for TNF was dose-dependent and related to the time of administration after TNF stimulation. In addition, TBP I inhibited membrane-bound TNF induced activation of human brain MVEC, but the concentration required was about 10-fold higher than that for soluble TNF. These results indicate a therapeutic potential for TBP I in diseases of the central nervous system associated with TNF overproduction.

KW - Adhesion molecules

KW - Brain microvascular endothelial cells

KW - Cytoadherence

KW - Cytotoxicity

KW - TNF

KW - TNF binding protein I

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ER -

Modulation of soluble and membrane-bound TNF-induced phenotypic and functional changes of human brain microvascular endothelial cells by recombinant TNF binding protein I

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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