TY - JOUR
T1 - Molecular cloning of the human placental folate transporter
AU - Prasad, Puttur D.
AU - Ramamoorthy, Sammanda
AU - Lei Bach, Frederick H.
AU - Ganapathy, Vadivel
PY - 1995/1/17
Y1 - 1995/1/17
N2 - We have isolated a cDNA clone from a human placental library which codes for a functional folate transporter (hFOLT). An RT-PCR product obtained from mouse intestinal mRNA using primers specific for the mouse folate transporter cDNA was used to screen the cDNA library. The hFOLT cDNA predicts a protein of 590 amino acids with twelve potential transmembrane domains. The amino acid sequence has 65% identity to the mouse and hamster folate transporters. When transfected into COS-1 and HeLa cells, the hFOLT cDNA causes a significant increase in the uptake of 5-methyltetrahydrofolate. The mRNA transcripts hybridizing to the hFOLT cDNA are detectable in the placenta and liver and also in several cell lines of human origin. The size of the principal transcript is 2.7 kb. This constitutes the first report on the cloning of a vitamin transporter from a human tissue.
AB - We have isolated a cDNA clone from a human placental library which codes for a functional folate transporter (hFOLT). An RT-PCR product obtained from mouse intestinal mRNA using primers specific for the mouse folate transporter cDNA was used to screen the cDNA library. The hFOLT cDNA predicts a protein of 590 amino acids with twelve potential transmembrane domains. The amino acid sequence has 65% identity to the mouse and hamster folate transporters. When transfected into COS-1 and HeLa cells, the hFOLT cDNA causes a significant increase in the uptake of 5-methyltetrahydrofolate. The mRNA transcripts hybridizing to the hFOLT cDNA are detectable in the placenta and liver and also in several cell lines of human origin. The size of the principal transcript is 2.7 kb. This constitutes the first report on the cloning of a vitamin transporter from a human tissue.
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U2 - 10.1006/bbrc.1995.1096
DO - 10.1006/bbrc.1995.1096
M3 - Article
C2 - 7826387
AN - SCOPUS:0028926410
SN - 0006-291X
VL - 206
SP - 681
EP - 687
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -