Molecular detection of B-cell neoplasms by specific DNA methylation biomarkers

Michael X. Wang, Huan You Wang, Xiaohui Zhao, Nalluri Srilatha, Dali Zheng, Huidong Shi, Jie Ning, Deiter J. Duff, Kristen H. Taylor, Barbara A. Gruner, Charles W. Caldwell

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


A novel, easy to perform PCR-based method employing specific DNA methylation biomarkers to detect Bcell neoplasms in a variety of B-cell lines and B lymphoblastic leukemia (B-ALL) patient specimens has been developed. This method detects as few as 5 B-ALL cells, or 1 B-ALL cell in 1,000,000 normal background blood cells using a single marker, DLC-1 gene CpG island (CGI) methylation. By adding two additional markers PCDHGA12 and RPIB9, over 80% of B-ALL cases were detected in patients' bone marrow and/or peripheral blood specimens. We have traced clinical B-ALL cases up to 10 years retrospectively and the DLC-1 methylation is correlated with patient clinical status. Thus, this epigenetic-based molecular method demonstrates its potential use in the diagnosis of B-cell neoplasia, in addition to traditional approach such as clinical features, morphology, immunophenotype, and genetic analysis.

Original languageEnglish (US)
Pages (from-to)265-279
Number of pages15
JournalInternational Journal of Clinical and Experimental Pathology
Issue number3
StatePublished - 2010


  • B lymphoblastic leukemia
  • DNA methylation biomarker
  • Mature B-cell neoplasms

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology


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