Abstract
Myeloid cells, specifically microglia and macrophages, are activated in retinal diseases and can improve or worsen retinopathy outcomes based on their inflammatory phenotype. However, assessing the myeloid cell response after retinal injury in mice remains challenging due to the small tissue size and the challenges of distinguishing microglia from infiltrating macrophages. In this protocol paper, we describe a flow cytometry-based protocol to assess retinal microglia/macrophage and their inflammatory phenotype after injury. The protocol is amenable to the incorporation of other markers of interest to other researchers.
Original language | English (US) |
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Article number | e4745 |
Journal | Bio-protocol |
Volume | 13 |
Issue number | 16 |
DOIs | |
State | Published - Aug 20 2023 |
Externally published | Yes |
Keywords
- Flow cytometry
- Macrophages
- Microglia
- Mouse retina
- Myeloid cells
- Retinopathy
ASJC Scopus subject areas
- General Neuroscience
- General Biochemistry, Genetics and Molecular Biology
- General Immunology and Microbiology
- Plant Science