@article{c95945a5df5847a7815d99162a0ae13c,
title = "Multifunctional Nanomachinery for Enhancement of Bone Healing",
abstract = "The visionary idea that RNA adopts nonbiological roles in today's nanomaterial world has been nothing short of phenomenal. These RNA molecules have ample chemical functionality and self-assemble to form distinct nanostructures in response to external stimuli. They may be combined with inorganic materials to produce nanomachines that carry cargo to a target site in a controlled manner and respond dynamically to environmental changes. Comparable to biological cells, programmed RNA nanomachines have the potential to replicate bone healing in vitro. Here, an RNA–biomineral nanomachine is developed, which accomplishes intrafibrillar and extrafibrillar mineralization of collagen scaffolds to mimic bone formation in vitro. Molecular dynamics simulation indicates that noncovalent hydrogen bonding provides the energy source that initiates self-assembly of these nanomachines. Incorporation of the RNA–biomineral nanomachines into collagen scaffolds in vivo creates an osteoinductive microenvironment within a bone defect that is conducive to rapid biomineralization and osteogenesis. Addition of RNA-degrading enzymes into RNA–biomineral nanomachines further creates a stop signal that inhibits unwarranted bone formation in tissues. The potential of RNA in building functional nanostructures has been underestimated in the past. The concept of RNA–biomineral nanomachines participating in physiological processes may transform the nanoscopic world of life science.",
keywords = "amorphous calcium phosphate, biomineralization, bone healing, nanomachines, RNA",
author = "Shen, {Min juan} and Wang, {Chen yu} and Hao, {Dong xiao} and Hao, {Jia xin} and Zhu, {Yi fei} and Han, {Xiao xiao} and Lige Tonggu and Chen, {Ji hua} and Kai Jiao and Tay, {Franklin R.} and Niu, {Li na}",
note = "Funding Information: M.j.S. and C.‐y.W. are contributed equally to this work. The authors thank S.Z. for assistance in the molecular dynamics simulations. This work was supported by Grant Nos. 81870805, 81870787, 82170978, and 81720108011 from National Natural Science Foundation of China, Grant No. 2020TD‐033 from the Shaanxi Key Scientific and Technological Innovation Team, the Youth Innovation Team of Shaanxi Universities and by Grant No. 2021JC‐34 from Distinguished Young Scientists Foundation of Shaanxi Province. Four‐week‐old male C57BL/6J mice were purchased from the Laboratory Animal and Welfare Committee of the Air Force Military Medical University. All surgical procedures were performed in compliance with the guidelines of the Laboratory Animal and Welfare Committee. Human caries‐free third molars were extracted from the patients who gave informed consent prior to extraction. The protocol was approved by the ethics committee of the Air Force Medical University. Funding Information: M.j.S. and C.-y.W. are contributed equally to this work. The authors thank S.Z. for assistance in the molecular dynamics simulations. This work was supported by Grant Nos. 81870805, 81870787, 82170978, and 81720108011 from National Natural Science Foundation of China, Grant No. 2020TD-033 from the Shaanxi Key Scientific and Technological Innovation Team, the Youth Innovation Team of Shaanxi Universities and by Grant No. 2021JC-34 from Distinguished Young Scientists Foundation of Shaanxi Province. Four-week-old male C57BL/6J mice were purchased from the Laboratory Animal and Welfare Committee of the Air Force Military Medical University. All surgical procedures were performed in compliance with the guidelines of the Laboratory Animal and Welfare Committee. Human caries-free third molars were extracted from the patients who gave informed consent prior to extraction. The protocol was approved by the ethics committee of the Air Force Medical University. Publisher Copyright: {\textcopyright} 2022 Wiley-VCH GmbH",
year = "2022",
month = mar,
day = "3",
doi = "10.1002/adma.202107924",
language = "English (US)",
volume = "34",
journal = "Advanced Materials",
issn = "0935-9648",
publisher = "Wiley-VCH Verlag",
number = "9",
}