Mutant p53 in cell adhesion and motility

W. Andrew Yeudall, Katharine H. Wrighton, Sumitra Deb

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Scopus citations


Pro-oncogenic properties of mutant p53 were investigated with the aid of migration assays, adhesion assays, and soft agar growth assays using cells stably expressing gain-of-function p53 mutants. To determine cell migration, "wound-healing" (scratch) assays and haptotactic (chamber) assays were used. H1299 cells expressing mutant p53 were found to migrate more rapidly than cells transfected with empty vector alone. Results from both types of migration assay were broadly similar. Migratory ability differed for different p53 mutants, suggesting allele-specific effects. Cells expressing p53 mutants also showed enhanced adhesion to extracellular matrix compare to controls. Furthermore, stable transfection of mutant p53-H179L into NIH3T3 fibroblasts was sufficient to allow anchorage-independent growth in soft agar.

Original languageEnglish (US)
Title of host publicationp53 Protocols
PublisherHumana Press Inc.
Number of pages12
ISBN (Print)9781627032353
StatePublished - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Adhesion
  • Anchorage independence
  • Chemotaxis
  • Extracellular matrix
  • Gain-of-function
  • Migration
  • Motility

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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