TY - JOUR
T1 - Mutation of PEB4 alters the outer membrane protein profile of Campylobacter jejuni
AU - Rathbun, Kimberly M.
AU - Thompson, Stuart A.
PY - 2009/11
Y1 - 2009/11
N2 - Campylobacter jejuni is a significant cause of human gastroenteritis worldwide. In an attempt to further define bacterial factors that influence infectivity, Cj0596 was identified as playing a role in C. jejuni virulence. Cj0596 is a periplasmic chaperone that is similar to proteins involved in outer membrane protein (OMP) folding in other bacteria. Mutation of cj0596 caused an alteration in the levels of eight OMPs, compared with wild-type bacteria. Replacement of the cj0596 mutation with the wild-type cj0596 gene restored a wild-type OMP profile. The altered OMP profile in the cj0596 mutant was accompanied by significant changes in several virulence properties, including an increase in the ability to autoagglutinate, increased susceptibility to several antimicrobial agents, and increased biofilm formation. In summary, mutation of cj0596 alters the C. jejuni OMP profile and leads to changes in OMP-related phenotypes involved in C. jejuni pathogenesis.
AB - Campylobacter jejuni is a significant cause of human gastroenteritis worldwide. In an attempt to further define bacterial factors that influence infectivity, Cj0596 was identified as playing a role in C. jejuni virulence. Cj0596 is a periplasmic chaperone that is similar to proteins involved in outer membrane protein (OMP) folding in other bacteria. Mutation of cj0596 caused an alteration in the levels of eight OMPs, compared with wild-type bacteria. Replacement of the cj0596 mutation with the wild-type cj0596 gene restored a wild-type OMP profile. The altered OMP profile in the cj0596 mutant was accompanied by significant changes in several virulence properties, including an increase in the ability to autoagglutinate, increased susceptibility to several antimicrobial agents, and increased biofilm formation. In summary, mutation of cj0596 alters the C. jejuni OMP profile and leads to changes in OMP-related phenotypes involved in C. jejuni pathogenesis.
KW - Campylobacter jejuni
KW - Cj0596
KW - PPIase
UR - http://www.scopus.com/inward/record.url?scp=70350220740&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70350220740&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.2009.01795.x
DO - 10.1111/j.1574-6968.2009.01795.x
M3 - Article
C2 - 19824902
AN - SCOPUS:70350220740
SN - 0378-1097
VL - 300
SP - 188
EP - 194
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2
ER -