TY - JOUR
T1 - Nail and Bone Surface as Biomarkers for Acute Fluoride Exposure in Rats
AU - Rabelo Buzalaf, Marília Afonso
AU - Caroselli, Elide Escolástico
AU - Cardoso De Oliveira, Rodrigo
AU - Granjeiro, José Mauro
AU - Whitford, Gary M.
N1 - Funding Information:
This work was supported by FAPESP (Grants n. 00/00826-6 and 01/07967-7). We thank Dr. Jos~ Roberto Pereira Lauris for the statistical analysis. We thank the students of the Laboratory of Biochemistry, Department of Biological Sciences, Bauru Dental School, University of S~.o Paulo, especially Tatiana, Vanessa, William, Maria, Everdan, Renato, and Gislene, for their valuable collaboration.
PY - 2004
Y1 - 2004
N2 - When acute exposure to fluoride is thought to be the cause of death, confirmation often depends on the analysis of some body fluid or tissue. The aim of this study was to evaluate the use of nails and the periosteal surface of bone as indicators of acute exposure to fluoride. Six groups of rats were given a single oral dose of fluoride (50 mg/kg body weight), while the control group was given deionized water. The rats were killed at 2, 4, 8, 16, 24, and 48 h after fluoride administration. Plasma and nails (the proximal halves) were collected and analyzed for fluoride with an ion-specific electrode after hexamethyldisiloxane-facilitated diffusion. A circular area of the femur (4.52 mm2) was etched with 0.5M HCl for 15 s, and, after the addition of a buffer, the solution was analyzed with an ion-specific electrode. Peak plasma concentration occurred at 2 h, followed by progressively declining concentrations. Peak nail fluoride concentrations occurred at 8 h. The mean nail concentrations at 8, 16, and 24 h were significantly higher than that of the control group. Bone surface concentrations were significantly higher than that of the control group at 4 h and thereafter. Thus, the proximal portion of nails and bone surface are suitable biomarkers for acute fluoride exposure in rats.
AB - When acute exposure to fluoride is thought to be the cause of death, confirmation often depends on the analysis of some body fluid or tissue. The aim of this study was to evaluate the use of nails and the periosteal surface of bone as indicators of acute exposure to fluoride. Six groups of rats were given a single oral dose of fluoride (50 mg/kg body weight), while the control group was given deionized water. The rats were killed at 2, 4, 8, 16, 24, and 48 h after fluoride administration. Plasma and nails (the proximal halves) were collected and analyzed for fluoride with an ion-specific electrode after hexamethyldisiloxane-facilitated diffusion. A circular area of the femur (4.52 mm2) was etched with 0.5M HCl for 15 s, and, after the addition of a buffer, the solution was analyzed with an ion-specific electrode. Peak plasma concentration occurred at 2 h, followed by progressively declining concentrations. Peak nail fluoride concentrations occurred at 8 h. The mean nail concentrations at 8, 16, and 24 h were significantly higher than that of the control group. Bone surface concentrations were significantly higher than that of the control group at 4 h and thereafter. Thus, the proximal portion of nails and bone surface are suitable biomarkers for acute fluoride exposure in rats.
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U2 - 10.1093/jat/28.4.249
DO - 10.1093/jat/28.4.249
M3 - Article
C2 - 15189675
AN - SCOPUS:2342478540
SN - 0146-4760
VL - 28
SP - 249
EP - 252
JO - Journal of Analytical Toxicology
JF - Journal of Analytical Toxicology
IS - 4
ER -