Abstract
We describe a novel technique combining precise organelle microextraction with deposition and matrix-assisted laser desorption/ionization (MALDI) for a rapid, minimally invasive mass spectrometry (MS) analysis of single organelles from living cells. A dual-positioner nanomanipulator workstation was utilized for both extraction of organelle content and precise co-deposition of analyte and matrix solution for MALDI-direct organelle mass spectrometry (DOMS) analysis. Here, the triacylglycerol (TAG) profiles of single lipid droplets from 3T3-L1 adipocytes were acquired and results validated with nanoelectrospray ionization (NSI) MS. The results demonstrate the utility of the MALDI-DOMS technique as it enabled longer mass analysis time, higher ionization efficiency, MS imaging of the co-deposited spot, and subsequent MS/MS capabilities of localized lipid content in comparison to NSI-DOMS. This method provides selective organellar resolution, which complements current biochemical analyses and prompts for subsequent subcellular studies to be performed where limited samples and analyte volume are of concern.
Original language | English (US) |
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Pages (from-to) | 187-193 |
Number of pages | 7 |
Journal | Journal of the American Society for Mass Spectrometry |
Volume | 27 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1 2016 |
Externally published | Yes |
Keywords
- Adipocytes
- Lipids
- MALDI
- Nanomanipulation
- Organelle
- Single cell
ASJC Scopus subject areas
- Structural Biology
- Spectroscopy