Abstract
Neuronal growth-inhibitory factor (GIF), also named metallothionein-3, inhibits the outgrowth of neuronal cells. Recent studies on the structure of human GIF, carried out using NMR and molecular dynamics simulation techniques, have been summarized. By studying a series of protein-engineered mutants of GIF, we showed that the bioactivity of GIF is modulated by multiple factors, including the unique TCPCP motif-induced characteristic conformation, the solvent accessibility and dynamics of the metal-thiolate cluster, and the domain-domain interactions.
Original language | English (US) |
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Pages (from-to) | 2912-2920 |
Number of pages | 9 |
Journal | FEBS Journal |
Volume | 277 |
Issue number | 14 |
DOIs | |
State | Published - Jul 2010 |
Externally published | Yes |
Keywords
- metallothionein (MT)
- mutation
- neuronal growth-inhibitory factor (GIF)
- structure-function relationship
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology