Neutrophil arrest by LFA-1 activation

Craig T. Lefort, Klaus Ley

Research output: Contribution to journalReview articlepeer-review

44 Scopus citations


Lymphocyte function-associated antigen-1 (LFA-1) is a heterodimeric integrin consisting of αL (genename, Itgal)andβ2 (genename, Itgb2)subunitsexpressedinallleukocytes. LFA-1 isessentialforneutrophilrecruitmenttoinflamedtissue.ActivationofLFA-1bychemokines allows neutrophils and other leukocytes to undergo arrest, resulting in firm adhesion on endothelia expressing intercellular adhesion molecules (ICAMs). In mice, CXCR2 is the primary chemokine receptor involved in triggering neutrophil arrest, and it does so through "inside-out" activation of LFA-1. CXCR2 signaling induces changes in LFA-1 conformation that are coupled to affinity upregulation of the ligand-binding headpiece (extended with open I domain). Unlike naïve lymphocytes, engagement of P-selectin glycoprotein ligand-1 (PSGL-1) on neutrophils stimulates a slow rolling behavior that is mediated by LFA-1 in a distinct activation state (extended with closed I domain). How inside-out signaling cas-cades regulate the structure and function of LFA-1 is being studied using flow chambers, intravital microscopy, and flow cytometry for ligand and reporter antibody binding. Here, we review how LFA-1 activation is regulated by cellular signaling and ligand binding. Two FERM domain-containing proteins, talin-1 and Kindlin-3, are critical integrin co-activators andhavedistinctrolesintheinductionofLFA-1conformationalrearrangements.Thisreview integrates these new results into existing models of LFA-1 activation.

Original languageEnglish (US)
Article numberArticle 157
JournalFrontiers in immunology
Issue numberJUN
StatePublished - 2012
Externally publishedYes


  • Chemokine
  • Inflammation
  • Integrin
  • Lfa-1
  • Neutrophil

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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