TY - JOUR
T1 - Novel anti-carcinogenic activity of an organosulfide from garlic
T2 - Inhibition of H-ras oncogene transformed tumor growth in vivo by diallyl disulfide is associated with inhibition of p21(H-ras) processing
AU - Singh, Shivendra V.
AU - Mohan, Rajiv R.
AU - Agarwal, Rajesh
AU - Benson, Patrick J.
AU - Hu, Xun
AU - Rudy, Maura A.
AU - Xia, Hong
AU - Katoh, Arthur
AU - Srivastava, Sanjay K.
AU - Mukhtar, Hasan
AU - Gupta, Vicram
AU - Zaren, Howard A.
N1 - Funding Information:
The authors thank Dr. Frank D’Amico, Duquesne University, for assistance in the statistical evaluation of tumor data. This work was supported, in part, by USPHS Grants CA 55589 (S.V.S.) and CA 64514 (R.A.), awarded by the National Cancer Institute. The financial support (to S.V.S.) from Pittsburgh Mercy Foundation (Jacob A. and Frieda M. Hunkele Charitable Fund and James Finlay Oncology Research Fund) is also acknowledged.
PY - 1996/8/14
Y1 - 1996/8/14
N2 - In this study, we report a novel anticarcinogenic activity of an organosulfur compound from garlic, diallyl disulfide (DADS). DADS treatment significantly inhibited the growth of H-ras oncogene transformed tumors in nude mice. As compared to controls, the appearance of tumors was also delayed markedly by oral administration of DADS. The inhibition of tumor growth by DADS treatment correlated with the inhibition of p21(H-ras) membrane association in the tumor tissue. The levels of membrane associated p21(H-ras) were markedly lower in the tumor tissues of DADS treated mice as compared to controls. An opposite trend, however, was evident for cytosolic p21(H-ras). Furthermore, DADS treatment resulted in a significant inhibition of hepatic as well as tumoral 3-hydroxy-3-methylglutaryl coenzyme A reductase activity. These results indicate that DADS suppresses the growth of H-ras oncogene transformed tumors in nude mice by inhibiting the membrane association of tumoral p21(H-ras).
AB - In this study, we report a novel anticarcinogenic activity of an organosulfur compound from garlic, diallyl disulfide (DADS). DADS treatment significantly inhibited the growth of H-ras oncogene transformed tumors in nude mice. As compared to controls, the appearance of tumors was also delayed markedly by oral administration of DADS. The inhibition of tumor growth by DADS treatment correlated with the inhibition of p21(H-ras) membrane association in the tumor tissue. The levels of membrane associated p21(H-ras) were markedly lower in the tumor tissues of DADS treated mice as compared to controls. An opposite trend, however, was evident for cytosolic p21(H-ras). Furthermore, DADS treatment resulted in a significant inhibition of hepatic as well as tumoral 3-hydroxy-3-methylglutaryl coenzyme A reductase activity. These results indicate that DADS suppresses the growth of H-ras oncogene transformed tumors in nude mice by inhibiting the membrane association of tumoral p21(H-ras).
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U2 - 10.1006/bbrc.1996.1226
DO - 10.1006/bbrc.1996.1226
M3 - Article
C2 - 8753815
AN - SCOPUS:0030583242
SN - 0006-291X
VL - 225
SP - 660
EP - 665
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -