Observation of the resting and pulsed states of cytochrome c oxidase in electrode-supported lipid bilayer membranes

James D. Burgess; Melissa C. Rhoten; Fred M. Hawkridge

doi:10.1021/ja974275z

Observation of the resting and pulsed states of cytochrome c oxidase in electrode-supported lipid bilayer membranes

James D. Burgess, Melissa C. Rhoten, Fred M. Hawkridge

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Cytochrome c oxidase immobilized in lipid bilayer membranes on gold electrodes mediates electron transfer between reduced cytochrome c in solution and the electrode. Under flow conditions, fixed potential amperometry of anaerobic solutions of reduced cytochrome c shows changes in the rate of enzyme mediated electron transfer from cytochrome c to oxidase modified electrodes. These data are consistent with literature that describes both activation of the resting oxidase state to the pulsed oxidase state and subsequent decay of the pulsed oxidase state to the resting oxidase state.

Original language	English (US)
Pages (from-to)	4488-4491
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	120
Issue number	18
DOIs	https://doi.org/10.1021/ja974275z
State	Published - May 13 1998
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

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abstract = "Cytochrome c oxidase immobilized in lipid bilayer membranes on gold electrodes mediates electron transfer between reduced cytochrome c in solution and the electrode. Under flow conditions, fixed potential amperometry of anaerobic solutions of reduced cytochrome c shows changes in the rate of enzyme mediated electron transfer from cytochrome c to oxidase modified electrodes. These data are consistent with literature that describes both activation of the resting oxidase state to the pulsed oxidase state and subsequent decay of the pulsed oxidase state to the resting oxidase state.",

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year = "1998",

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AU - Burgess, James D.

AU - Rhoten, Melissa C.

AU - Hawkridge, Fred M.

PY - 1998/5/13

Y1 - 1998/5/13

N2 - Cytochrome c oxidase immobilized in lipid bilayer membranes on gold electrodes mediates electron transfer between reduced cytochrome c in solution and the electrode. Under flow conditions, fixed potential amperometry of anaerobic solutions of reduced cytochrome c shows changes in the rate of enzyme mediated electron transfer from cytochrome c to oxidase modified electrodes. These data are consistent with literature that describes both activation of the resting oxidase state to the pulsed oxidase state and subsequent decay of the pulsed oxidase state to the resting oxidase state.

AB - Cytochrome c oxidase immobilized in lipid bilayer membranes on gold electrodes mediates electron transfer between reduced cytochrome c in solution and the electrode. Under flow conditions, fixed potential amperometry of anaerobic solutions of reduced cytochrome c shows changes in the rate of enzyme mediated electron transfer from cytochrome c to oxidase modified electrodes. These data are consistent with literature that describes both activation of the resting oxidase state to the pulsed oxidase state and subsequent decay of the pulsed oxidase state to the resting oxidase state.

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Observation of the resting and pulsed states of cytochrome c oxidase in electrode-supported lipid bilayer membranes

Abstract

ASJC Scopus subject areas

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