Optical absorbance and optical rotatory dispersion studies on calf thymus nucleohistone

Dorothy Y.H. Tuan; James Bonner

doi:10.1016/0022-2836(69)90209-5

Optical absorbance and optical rotatory dispersion studies on calf thymus nucleohistone

Dorothy Y.H. Tuan, James Bonner

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

99 Scopus citations

Abstract

Optical absorption and optical rotatory dispersion studies of native nucleohistone and of nucleohistone fully or partially and selectively dissociated into DNA and histone, show that (1) the conformation of whole histone mixtures as well as of individual histone fractions is highly ionic strength dependent and that the conformation of histone in association with DNA approximates that of free histone in solutions of high ionic strength; (2) the conformation of DNA in native nucleohistone is altered as compared to free DNA; and (3) neither lysinerich histone I nor non-histone protein is responsible for the conformational characteristics of DNA in nucleohistone.

Original language	English (US)
Pages (from-to)	59-76
Number of pages	18
Journal	Journal of Molecular Biology
Volume	45
Issue number	1
DOIs	https://doi.org/10.1016/0022-2836(69)90209-5
State	Published - Oct 14 1969

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1016/0022-2836(69)90209-5

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title = "Optical absorbance and optical rotatory dispersion studies on calf thymus nucleohistone",

abstract = "Optical absorption and optical rotatory dispersion studies of native nucleohistone and of nucleohistone fully or partially and selectively dissociated into DNA and histone, show that (1) the conformation of whole histone mixtures as well as of individual histone fractions is highly ionic strength dependent and that the conformation of histone in association with DNA approximates that of free histone in solutions of high ionic strength; (2) the conformation of DNA in native nucleohistone is altered as compared to free DNA; and (3) neither lysinerich histone I nor non-histone protein is responsible for the conformational characteristics of DNA in nucleohistone.",

author = "Tuan, {Dorothy Y.H.} and James Bonner",

note = "Funding Information: We thank ProfessorN orman Davidson and Dr Roger Chalkley for helpful disoussions and suggeatione. This work was supportedi n part by U. S. Publia Health Service grant CM 13782and the M&&m Fund of the Celifornie Institute of Technology.",

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doi = "10.1016/0022-2836(69)90209-5",

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T1 - Optical absorbance and optical rotatory dispersion studies on calf thymus nucleohistone

AU - Tuan, Dorothy Y.H.

AU - Bonner, James

N1 - Funding Information: We thank ProfessorN orman Davidson and Dr Roger Chalkley for helpful disoussions and suggeatione. This work was supportedi n part by U. S. Publia Health Service grant CM 13782and the M&&m Fund of the Celifornie Institute of Technology.

PY - 1969/10/14

Y1 - 1969/10/14

N2 - Optical absorption and optical rotatory dispersion studies of native nucleohistone and of nucleohistone fully or partially and selectively dissociated into DNA and histone, show that (1) the conformation of whole histone mixtures as well as of individual histone fractions is highly ionic strength dependent and that the conformation of histone in association with DNA approximates that of free histone in solutions of high ionic strength; (2) the conformation of DNA in native nucleohistone is altered as compared to free DNA; and (3) neither lysinerich histone I nor non-histone protein is responsible for the conformational characteristics of DNA in nucleohistone.

AB - Optical absorption and optical rotatory dispersion studies of native nucleohistone and of nucleohistone fully or partially and selectively dissociated into DNA and histone, show that (1) the conformation of whole histone mixtures as well as of individual histone fractions is highly ionic strength dependent and that the conformation of histone in association with DNA approximates that of free histone in solutions of high ionic strength; (2) the conformation of DNA in native nucleohistone is altered as compared to free DNA; and (3) neither lysinerich histone I nor non-histone protein is responsible for the conformational characteristics of DNA in nucleohistone.

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Optical absorbance and optical rotatory dispersion studies on calf thymus nucleohistone

Abstract

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