Parathyroid hormone effects on signaling pathways in endothelial cells vary with peptide concentration

Doug Throckmorton; Doris Kurscheid-Reich; Oscar R. Rosales; Jose Rodriguez-Commes; Raquel Lopez; Bauer Sumpio; Qing Zhong; Ke Hong Ding; Richard McCarthy; Paula Q. Barrett; Carlos M. Isales

doi:10.1016/S0196-9781(01)00582-4

Parathyroid hormone effects on signaling pathways in endothelial cells vary with peptide concentration

Doug Throckmorton, Doris Kurscheid-Reich, Oscar R. Rosales, Jose Rodriguez-Commes, Raquel Lopez, Bauer Sumpio, Qing Zhong, Ke Hong Ding, Richard McCarthy, Paula Q. Barrett, Carlos M. Isales

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

We have previously reported that parathyroid hormone (PTH) has specific effects on a human umbilical vein endothelial cell line. Further studies were performed to characterize the signaling cascades initiated by PTH. We report that PTH induced the appearance of voltage sensitive calcium channels. Furthermore, PTH increased ceramide but not diacylglycerol content. Since elevations in [Ca²⁺]_i and phospholipid turnover are signals for the activation of protein kinase C (PKC), the cells were screened for PKC isoforms. PTH induced a redistribution of the PKCε to the particulate fractions of cell homogenates. In summary, PTH induced PKC translocation through a calcium-phospholipid pathway in an endothelial cell line.

Original language	English (US)
Pages (from-to)	79-85
Number of pages	7
Journal	Peptides
Volume	23
Issue number	1
DOIs	https://doi.org/10.1016/S0196-9781(01)00582-4
State	Published - 2002

Keywords

Calcium channels
Ceramide
Diacylglycerol
PTH
Protein kinase C

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

Access to Document

10.1016/S0196-9781(01)00582-4

Cite this

@article{a23ed52044a64fa5abcea7eade778672,

title = "Parathyroid hormone effects on signaling pathways in endothelial cells vary with peptide concentration",

abstract = "We have previously reported that parathyroid hormone (PTH) has specific effects on a human umbilical vein endothelial cell line. Further studies were performed to characterize the signaling cascades initiated by PTH. We report that PTH induced the appearance of voltage sensitive calcium channels. Furthermore, PTH increased ceramide but not diacylglycerol content. Since elevations in [Ca2+]i and phospholipid turnover are signals for the activation of protein kinase C (PKC), the cells were screened for PKC isoforms. PTH induced a redistribution of the PKCε to the particulate fractions of cell homogenates. In summary, PTH induced PKC translocation through a calcium-phospholipid pathway in an endothelial cell line.",

keywords = "Calcium channels, Ceramide, Diacylglycerol, PTH, Protein kinase C",

author = "Doug Throckmorton and Doris Kurscheid-Reich and Rosales, {Oscar R.} and Jose Rodriguez-Commes and Raquel Lopez and Bauer Sumpio and Qing Zhong and Ding, {Ke Hong} and Richard McCarthy and Barrett, {Paula Q.} and Isales, {Carlos M.}",

year = "2002",

doi = "10.1016/S0196-9781(01)00582-4",

language = "English (US)",

volume = "23",

pages = "79--85",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "1",

}

TY - JOUR

T1 - Parathyroid hormone effects on signaling pathways in endothelial cells vary with peptide concentration

AU - Throckmorton, Doug

AU - Kurscheid-Reich, Doris

AU - Rosales, Oscar R.

AU - Rodriguez-Commes, Jose

AU - Lopez, Raquel

AU - Sumpio, Bauer

AU - Zhong, Qing

AU - Ding, Ke Hong

AU - McCarthy, Richard

AU - Barrett, Paula Q.

AU - Isales, Carlos M.

PY - 2002

Y1 - 2002

N2 - We have previously reported that parathyroid hormone (PTH) has specific effects on a human umbilical vein endothelial cell line. Further studies were performed to characterize the signaling cascades initiated by PTH. We report that PTH induced the appearance of voltage sensitive calcium channels. Furthermore, PTH increased ceramide but not diacylglycerol content. Since elevations in [Ca2+]i and phospholipid turnover are signals for the activation of protein kinase C (PKC), the cells were screened for PKC isoforms. PTH induced a redistribution of the PKCε to the particulate fractions of cell homogenates. In summary, PTH induced PKC translocation through a calcium-phospholipid pathway in an endothelial cell line.

AB - We have previously reported that parathyroid hormone (PTH) has specific effects on a human umbilical vein endothelial cell line. Further studies were performed to characterize the signaling cascades initiated by PTH. We report that PTH induced the appearance of voltage sensitive calcium channels. Furthermore, PTH increased ceramide but not diacylglycerol content. Since elevations in [Ca2+]i and phospholipid turnover are signals for the activation of protein kinase C (PKC), the cells were screened for PKC isoforms. PTH induced a redistribution of the PKCε to the particulate fractions of cell homogenates. In summary, PTH induced PKC translocation through a calcium-phospholipid pathway in an endothelial cell line.

KW - Calcium channels

KW - Ceramide

KW - Diacylglycerol

KW - PTH

KW - Protein kinase C

UR - http://www.scopus.com/inward/record.url?scp=18244395885&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18244395885&partnerID=8YFLogxK

U2 - 10.1016/S0196-9781(01)00582-4

DO - 10.1016/S0196-9781(01)00582-4

M3 - Article

C2 - 11814621

AN - SCOPUS:18244395885

SN - 0196-9781

VL - 23

SP - 79

EP - 85

JO - Peptides

JF - Peptides

IS - 1

ER -

Parathyroid hormone effects on signaling pathways in endothelial cells vary with peptide concentration

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this