Abstract
A study was made of the partition and purification of α-amylase from a culture supernatant of Bacillus subtilis in the polyethylene glycol (PEG) - citrate aqueous two-phase system (ATPS). Factors that influenced the partition of the protein in this system, including the molecular weight of the PEG, the tie line length of ATPS, the pH value and the sodium chloride concentration, were investigated. Purification of α-amylase was attained with a purification factor (PF) of 1.8 and 90% yield at pH 6.0 in a PEG1000-citrate ATPS with short tie line length. By utilizing the salt-out effect of neutral salt, the purification of α-amylase was further improved to 2.0 of PF and 80% yield in a PEG3350-citrate ATPS with 4% sodium chloride.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3-7 |
| Number of pages | 5 |
| Journal | Bioprocess and Biosystems Engineering |
| Volume | 27 |
| Issue number | 1 |
| DOIs | |
| State | Published - Dec 2004 |
| Externally published | Yes |
Keywords
- Aqueous two-phase systems
- Purification
- α-Amylase
ASJC Scopus subject areas
- Biotechnology
- Bioengineering