Performance of a transcription-mediated-amplification HIV-1 RNA assay in pooled specimens

Nicola M. Zetola, Ann Mintie, Sally Liska, Ernest Wong, Joan Dunn Williams, Michael P. Busch, Jeffrey D. Klausner

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Background: Very limited data exist on the comparative performance of nucleic acid amplification tests (NATs) for the screening of pooled specimens for acute human immunodeficiency virus (HIV) infection. Study design: In this study, we compared a transcription-mediated-amplification assay (Procleix® HIV-1 Discriminatory Assay, [TMA]) with a branched DNA assay (Bayer Versant HIV-1 RNA 3.0 assay, [bDNA]). Results: After re-testing 1552 samples that were negative for HIV RNA by bDNA, we found one additional positive sample with the TMA assay. Conclusion: Our results suggest that TMA could potentially detect acute HIV infections missed by other technologies.

Original languageEnglish (US)
Pages (from-to)68-70
Number of pages3
JournalJournal of Clinical Virology
Issue number1
StatePublished - Sep 2007
Externally publishedYes


  • bDNA
  • HIV
  • Pooled testing
  • TMA

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases


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