Abstract
NO is crucial for endothelial function and vascular health. Plasma nitrite (NO2-) is the main oxidation product of NO and has been shown to reflect changes in eNOS activity. We hypothesized that plasma NO2- response to physical exercise stress along with physiological endothelial function would be reduced with increasing severity of vascular disease. Subject groups were: (a) risk factors but no vascular disease (RF); (b) Type 2 diabetes with no vascular disease (DM); (c) diagnosed peripheral arterial disease (PAD); and (d) DM + PAD. Venous blood was drawn at rest and 10 min following maximal exercise. Plasma samples were analyzed by reductive chemiluminescence. Brachial diameters were imaged prior to, during and following 5 min of forearm occlusion (BAFMD). There were no differences in resting plasma NO2- or BA diameters between groups. The PAD groups had lower age adjusted BAFMD responses (p ≤ 0.05). Within group analysis revealed an increase in NO2- in the RF group (+39.3%), no change in the DM (-15.51%), and a decrease in the PAD (-44.20%) and PAD + DM (-39.95%). This was maintained after adjusting for age and VO2peak (p ≤ 0.05). ΔNO2- and BAFMD were the strongest independent predictors of VO2peak in multivariate linear regression. These findings suggest ΔNO2- discriminates severity of cardiovascular disease risk, is related to endothelial function and predicts exercise capacity.
Original language | English (US) |
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Pages (from-to) | 231-237 |
Number of pages | 7 |
Journal | Nitric Oxide - Biology and Chemistry |
Volume | 20 |
Issue number | 4 |
DOIs | |
State | Published - Jun 1 2009 |
Externally published | Yes |
Keywords
- Cardiovascular disease
- Endothelium
- Exercise
- Nitric oxide
- Plasma nitrite
- VO
ASJC Scopus subject areas
- Biochemistry
- Physiology
- Clinical Biochemistry
- Cancer Research