Pro-apoptotic effect of fly ash leachates in hepatocytes of freshwater fish (Channa punctata Bloch)

Mehboob Ali; Shakilur Rahman; Hasibur Rehman; Kanchan Bhatia; Rizwan A. Ansari; Sheikh Raisuddin

doi:10.1016/j.tiv.2006.08.011

Pro-apoptotic effect of fly ash leachates in hepatocytes of freshwater fish (Channa punctata Bloch)

Mehboob Ali, Shakilur Rahman, Hasibur Rehman, Kanchan Bhatia, Rizwan A. Ansari, Sheikh Raisuddin

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The pro-apoptotic effect of fly ash leachates (FAL) was studied in the hepatocytes of an Indian freshwater fish, Channa punctata Bloch. Hepatocytes were exposed to different concentrations of '7-day' FAL for 24 and 48 h and various parameters of apoptosis were studied using standardized procedures. FAL-induced apoptosis in hepatocytes was indicated by cytological examination, DNA fragmentation and DNA laddering. The induction in cytochrome-c release, caspases 3, 7, 10 and 9 activities and lactate dehydrogenase level provide mechanistic platform for FAL-induced apoptosis. Cytological examination showed an unambiguous apoptotic effect of ash leachates in fish hepatocytes. Exposed hepatocytes also showed increased production of H₂O₂, superoxide ions and an increase in lipid peroxidation (LPO). The present study suggests a possible role of reactive oxygen species (ROS) in FAL-induced apoptosis in hepatocytes. Lactate dehydrogenase, LPO and apoptosis as biomarkers of cytotoxicity have recently been used for assessment of ecotoxicological impact of environmental chemicals. Our findings show that these biomarkers may also be used for evaluation of ecotoxicological impact of complex chemical mixture such as fly ash and its leachates.

Original language	English (US)
Pages (from-to)	63-71
Number of pages	9
Journal	Toxicology in Vitro
Volume	21
Issue number	1
DOIs	https://doi.org/10.1016/j.tiv.2006.08.011
State	Published - Feb 2007
Externally published	Yes

Keywords

Apoptosis
Biomarkers
Caspases
Fish hepatocytes
Fly ash leachates
Reactive oxygen species

ASJC Scopus subject areas

Toxicology

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10.1016/j.tiv.2006.08.011

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title = "Pro-apoptotic effect of fly ash leachates in hepatocytes of freshwater fish (Channa punctata Bloch)",

abstract = "The pro-apoptotic effect of fly ash leachates (FAL) was studied in the hepatocytes of an Indian freshwater fish, Channa punctata Bloch. Hepatocytes were exposed to different concentrations of '7-day' FAL for 24 and 48 h and various parameters of apoptosis were studied using standardized procedures. FAL-induced apoptosis in hepatocytes was indicated by cytological examination, DNA fragmentation and DNA laddering. The induction in cytochrome-c release, caspases 3, 7, 10 and 9 activities and lactate dehydrogenase level provide mechanistic platform for FAL-induced apoptosis. Cytological examination showed an unambiguous apoptotic effect of ash leachates in fish hepatocytes. Exposed hepatocytes also showed increased production of H2O2, superoxide ions and an increase in lipid peroxidation (LPO). The present study suggests a possible role of reactive oxygen species (ROS) in FAL-induced apoptosis in hepatocytes. Lactate dehydrogenase, LPO and apoptosis as biomarkers of cytotoxicity have recently been used for assessment of ecotoxicological impact of environmental chemicals. Our findings show that these biomarkers may also be used for evaluation of ecotoxicological impact of complex chemical mixture such as fly ash and its leachates.",

keywords = "Apoptosis, Biomarkers, Caspases, Fish hepatocytes, Fly ash leachates, Reactive oxygen species",

author = "Mehboob Ali and Shakilur Rahman and Hasibur Rehman and Kanchan Bhatia and Ansari, {Rizwan A.} and Sheikh Raisuddin",

note = "Funding Information: Financial support for this study from the Ministry of Environment and Forests, Government of India is acknowledged. Authors thank Dr. Ehsan A. Khan, Head of the Department for his interest in our research work.",

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AU - Ali, Mehboob

AU - Rahman, Shakilur

AU - Rehman, Hasibur

AU - Bhatia, Kanchan

AU - Ansari, Rizwan A.

AU - Raisuddin, Sheikh

N1 - Funding Information: Financial support for this study from the Ministry of Environment and Forests, Government of India is acknowledged. Authors thank Dr. Ehsan A. Khan, Head of the Department for his interest in our research work.

PY - 2007/2

Y1 - 2007/2

N2 - The pro-apoptotic effect of fly ash leachates (FAL) was studied in the hepatocytes of an Indian freshwater fish, Channa punctata Bloch. Hepatocytes were exposed to different concentrations of '7-day' FAL for 24 and 48 h and various parameters of apoptosis were studied using standardized procedures. FAL-induced apoptosis in hepatocytes was indicated by cytological examination, DNA fragmentation and DNA laddering. The induction in cytochrome-c release, caspases 3, 7, 10 and 9 activities and lactate dehydrogenase level provide mechanistic platform for FAL-induced apoptosis. Cytological examination showed an unambiguous apoptotic effect of ash leachates in fish hepatocytes. Exposed hepatocytes also showed increased production of H2O2, superoxide ions and an increase in lipid peroxidation (LPO). The present study suggests a possible role of reactive oxygen species (ROS) in FAL-induced apoptosis in hepatocytes. Lactate dehydrogenase, LPO and apoptosis as biomarkers of cytotoxicity have recently been used for assessment of ecotoxicological impact of environmental chemicals. Our findings show that these biomarkers may also be used for evaluation of ecotoxicological impact of complex chemical mixture such as fly ash and its leachates.

AB - The pro-apoptotic effect of fly ash leachates (FAL) was studied in the hepatocytes of an Indian freshwater fish, Channa punctata Bloch. Hepatocytes were exposed to different concentrations of '7-day' FAL for 24 and 48 h and various parameters of apoptosis were studied using standardized procedures. FAL-induced apoptosis in hepatocytes was indicated by cytological examination, DNA fragmentation and DNA laddering. The induction in cytochrome-c release, caspases 3, 7, 10 and 9 activities and lactate dehydrogenase level provide mechanistic platform for FAL-induced apoptosis. Cytological examination showed an unambiguous apoptotic effect of ash leachates in fish hepatocytes. Exposed hepatocytes also showed increased production of H2O2, superoxide ions and an increase in lipid peroxidation (LPO). The present study suggests a possible role of reactive oxygen species (ROS) in FAL-induced apoptosis in hepatocytes. Lactate dehydrogenase, LPO and apoptosis as biomarkers of cytotoxicity have recently been used for assessment of ecotoxicological impact of environmental chemicals. Our findings show that these biomarkers may also be used for evaluation of ecotoxicological impact of complex chemical mixture such as fly ash and its leachates.

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KW - Fly ash leachates

KW - Reactive oxygen species

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Pro-apoptotic effect of fly ash leachates in hepatocytes of freshwater fish (Channa punctata Bloch)

Abstract

Keywords

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