Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis

John C. Sutherland; Denise C. Monteleone; John G. Trunk; Paula V. Bennett; Betsy M. Sutherland

doi:10.1002/1522-2683()22:5<843::AID-ELPS843>3.0.CO;2-9

Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis

John C. Sutherland, Denise C. Monteleone, John G. Trunk, Paula V. Bennett, Betsy M. Sutherland

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

DNA damages that can be converted to single- or doubl strand breaks ca be quntified by separating DNA y gel electrophoreis and obtinin a uantitative image of the resuling distribution o DNA in the gel. We review hetheory of this metho and discuss its implementation, including the charge-coupled device (CCD) camera systems we developed to cquired images o fluorophore labeled DNA.

Original language	English (US)
Pages (from-to)	843-854
Number of pages	12
Journal	ELECTROPHORESIS
Volume	22
Issue number	5
DOIs	https://doi.org/10.1002/1522-2683()22:5<843::AID-ELPS843>3.0.CO;2-9
State	Published - 2001
Externally published	Yes

Keywords

Agarose gel electrophoresis
DNA damage and repair
Electronic imaging

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry

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10.1002/1522-2683()22:5<843::AID-ELPS843>3.0.CO;2-9

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title = "Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis",

abstract = "DNA damages that can be converted to single- or doubl strand breaks ca be quntified by separating DNA y gel electrophoreis and obtinin a uantitative image of the resuling distribution o DNA in the gel. We review hetheory of this metho and discuss its implementation, including the charge-coupled device (CCD) camera systems we developed to cquired images o fluorophore labeled DNA.",

keywords = "Agarose gel electrophoresis, DNA damage and repair, Electronic imaging",

author = "Sutherland, {John C.} and Monteleone, {Denise C.} and Trunk, {John G.} and Bennett, {Paula V.} and Sutherland, {Betsy M.}",

year = "2001",

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language = "English (US)",

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AU - Sutherland, John C.

AU - Monteleone, Denise C.

AU - Trunk, John G.

AU - Bennett, Paula V.

AU - Sutherland, Betsy M.

PY - 2001

Y1 - 2001

N2 - DNA damages that can be converted to single- or doubl strand breaks ca be quntified by separating DNA y gel electrophoreis and obtinin a uantitative image of the resuling distribution o DNA in the gel. We review hetheory of this metho and discuss its implementation, including the charge-coupled device (CCD) camera systems we developed to cquired images o fluorophore labeled DNA.

AB - DNA damages that can be converted to single- or doubl strand breaks ca be quntified by separating DNA y gel electrophoreis and obtinin a uantitative image of the resuling distribution o DNA in the gel. We review hetheory of this metho and discuss its implementation, including the charge-coupled device (CCD) camera systems we developed to cquired images o fluorophore labeled DNA.

KW - Agarose gel electrophoresis

KW - DNA damage and repair

KW - Electronic imaging

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AN - SCOPUS:0035064055

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Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis

Abstract

Keywords

ASJC Scopus subject areas

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