Rapid Method for Isolation and Staining of Bacterial Lipopolysaccharide

Ayman Al-Hendy; Paavo Toivanen; Mikael Skurnik

doi:10.1111/j.1348-0421.1991.tb01562.x

Rapid Method for Isolation and Staining of Bacterial Lipopolysaccharide

Ayman Al-Hendy, Paavo Toivanen, Mikael Skurnik

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Classically bacterial lipopolysaccharide (LPS) purification and silver staining take several days. We designed a simple and fast method for LPS isolation which when combined with silver staining using Pharmacia PhastSystem™ both can be completed in few hours. The purity of LPS isolated by this simple method may not be comparable to that by the phenol-water method hence we recommend this rapid isolation and staining procedures for simple and fast study of LPS patterns in gels.

Original language	English (US)
Pages (from-to)	331-333
Number of pages	3
Journal	Microbiology and Immunology
Volume	35
Issue number	4
DOIs	https://doi.org/10.1111/j.1348-0421.1991.tb01562.x
State	Published - 1991
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Immunology
Virology

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10.1111/j.1348-0421.1991.tb01562.x

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title = "Rapid Method for Isolation and Staining of Bacterial Lipopolysaccharide",

abstract = "Classically bacterial lipopolysaccharide (LPS) purification and silver staining take several days. We designed a simple and fast method for LPS isolation which when combined with silver staining using Pharmacia PhastSystem{\texttrademark} both can be completed in few hours. The purity of LPS isolated by this simple method may not be comparable to that by the phenol-water method hence we recommend this rapid isolation and staining procedures for simple and fast study of LPS patterns in gels.",

author = "Ayman Al-Hendy and Paavo Toivanen and Mikael Skurnik",

year = "1991",

doi = "10.1111/j.1348-0421.1991.tb01562.x",

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AU - Al-Hendy, Ayman

AU - Toivanen, Paavo

AU - Skurnik, Mikael

PY - 1991

Y1 - 1991

N2 - Classically bacterial lipopolysaccharide (LPS) purification and silver staining take several days. We designed a simple and fast method for LPS isolation which when combined with silver staining using Pharmacia PhastSystem™ both can be completed in few hours. The purity of LPS isolated by this simple method may not be comparable to that by the phenol-water method hence we recommend this rapid isolation and staining procedures for simple and fast study of LPS patterns in gels.

AB - Classically bacterial lipopolysaccharide (LPS) purification and silver staining take several days. We designed a simple and fast method for LPS isolation which when combined with silver staining using Pharmacia PhastSystem™ both can be completed in few hours. The purity of LPS isolated by this simple method may not be comparable to that by the phenol-water method hence we recommend this rapid isolation and staining procedures for simple and fast study of LPS patterns in gels.

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Rapid Method for Isolation and Staining of Bacterial Lipopolysaccharide

Abstract

ASJC Scopus subject areas

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