TY - JOUR
T1 - Serum glycoproteomic alterations in patients with diabetic retinopathy
AU - Sharma, Ashok
AU - Cox, James
AU - Glass, Joshua
AU - Lee, Tae Jin
AU - Kodeboyina, Sai Karthik
AU - Zhi, Wenbo
AU - Ulrich, Lane
AU - Lukowski, Zachary
AU - Sharma, Shruti
N1 - Funding Information:
Funding: This study is supported by the National Institutes of Health, National Eye Institute (Bethesda, MD, USA) grant no. R01-EY026936 awarded to Shruti Sharma.
Publisher Copyright:
© 2020 by the authors.
PY - 2020/9
Y1 - 2020/9
N2 - The precise molecular mechanisms of diabetic retinopathy (DR) pathogenesis are unclear, and treatment options are limited. There is an urgent need to discover and develop novel therapeutic targets for the treatment of this disease. Glycosylation is a post-translational modification that plays a critical role in determining protein structure, function, and stability. Recent studies have found that serum glycoproteomic changes are associated with the presence or progression of several inflammatory diseases. However, very little is known about the glycoproteomic changes associated with DR. In this study, glycoproteomic profiling of the serum of diabetic patients with and without DR was performed. A total of 15 glycopeptides from 11 glycoproteins were found to be significantly altered (5 upregulated and 10 downregulated) within the serum glycoproteome of DR patients. These glycoproteins are known to be involved in the maintenance of the extracellular matrix and complement system through peptidolytic activity or regulation.
AB - The precise molecular mechanisms of diabetic retinopathy (DR) pathogenesis are unclear, and treatment options are limited. There is an urgent need to discover and develop novel therapeutic targets for the treatment of this disease. Glycosylation is a post-translational modification that plays a critical role in determining protein structure, function, and stability. Recent studies have found that serum glycoproteomic changes are associated with the presence or progression of several inflammatory diseases. However, very little is known about the glycoproteomic changes associated with DR. In this study, glycoproteomic profiling of the serum of diabetic patients with and without DR was performed. A total of 15 glycopeptides from 11 glycoproteins were found to be significantly altered (5 upregulated and 10 downregulated) within the serum glycoproteome of DR patients. These glycoproteins are known to be involved in the maintenance of the extracellular matrix and complement system through peptidolytic activity or regulation.
KW - Diabetic retinopathy
KW - Glycoproteomics
KW - LC-MS/MS
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U2 - 10.3390/PROTEOMES8030025
DO - 10.3390/PROTEOMES8030025
M3 - Article
AN - SCOPUS:85092130438
SN - 2227-7382
VL - 8
JO - Proteomes
JF - Proteomes
IS - 3
M1 - 25
ER -
