Sialidase activity in nuclear membranes of rat brain

Megumi Saito, Christian L. Fronda, Robert K. Yu

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


A highly purified nuclear membrane preparation was obtained from adult rat brain and examined for sialidase activity using GM3, GD1a, GD1b, or N- acetylneuramin lactitol as the substrate. The nuclear membranes contained an appreciable level of sialidase activity; the specific activities toward GM3 and N-acetylneuramin lactitol were 20.5 and 23.8% of the activities in the total brain homogenate, respectively. The sialidase activity in nuclear membranes showed substrate specificity distinct from other membrane-bound sialidases localized in lysosomal membranes, synaptosomal plasma membranes, or myelin membranes. These results strongly suggest the existence of a sialidase activity associated with the nuclear membranes from rat brain.

Original languageEnglish (US)
Pages (from-to)2205-2208
Number of pages4
JournalJournal of Neurochemistry
Issue number5
StatePublished - May 1996
Externally publishedYes


  • Brain
  • Ganglioside
  • Glycoconjugate
  • Neuraminidase
  • Nuclear membrane
  • Sialidase

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience


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