TY - JOUR
T1 - Standardized flavonoid-rich Eugenia jambolana seed extract retards in vitro and in vivo LDL oxidation and expression of VCAM-1 and P-selectin in atherogenic rats
AU - Jadeja, Ravirajsinh N.
AU - Thouaojam, Menaka C.
AU - Sankhari, Jayantha M.
AU - Jain, Mahendra
AU - Devkar, Ranjitsinh V.
AU - Ramachandran, A. V.
N1 - Funding Information:
Acknowledgments Authors R.N.J and M.C.T acknowledge CSIR, New Delhi, and UGC, New Delhi, for financial assistant in the form of Senior Research Fellowship and RFSMS fellowship, respectively. Thanks are also due to Prof. Sarita Gupta, Head Department of Biochemistry, for providing experimental animals.
PY - 2012/3
Y1 - 2012/3
N2 - The present inventory evaluates anti-atherogenic potential of flavonoid-rich Eugenia jambolana seed extract (EJSE) against in vitro low-density lipoprotein (LDL) oxidation, foam cell formation, and atherogenic (ATH) diet-induced experimental atherosclerosis in rats. EJSE was able to prevent in vitro LDL oxidation and oxidized LDL-induced macrophage foam cell formation. Also, EJSE supplementation to ATH rats significantly minimized increment in serum markers of LDL oxidation. The ex vivo oxidation indices were also minimized in LDL of EJSE-treated animals. Microscopic evaluation of thoracic aorta of ATH + EJSE rats recorded minimal evidence of atheromatous plaque formation, accumulation of lipid laden macrophages, calcium deposition, and expression of cell adhesion molecules (vascular cell adhesion molecule-1 and P-selectin). This is the first scientific report that demonstrates anti-atherogenic potential of EJSE and warrants further evaluation at clinical level.
AB - The present inventory evaluates anti-atherogenic potential of flavonoid-rich Eugenia jambolana seed extract (EJSE) against in vitro low-density lipoprotein (LDL) oxidation, foam cell formation, and atherogenic (ATH) diet-induced experimental atherosclerosis in rats. EJSE was able to prevent in vitro LDL oxidation and oxidized LDL-induced macrophage foam cell formation. Also, EJSE supplementation to ATH rats significantly minimized increment in serum markers of LDL oxidation. The ex vivo oxidation indices were also minimized in LDL of EJSE-treated animals. Microscopic evaluation of thoracic aorta of ATH + EJSE rats recorded minimal evidence of atheromatous plaque formation, accumulation of lipid laden macrophages, calcium deposition, and expression of cell adhesion molecules (vascular cell adhesion molecule-1 and P-selectin). This is the first scientific report that demonstrates anti-atherogenic potential of EJSE and warrants further evaluation at clinical level.
KW - Eugenia jambolana
KW - Flavonoids
KW - LDL oxidation
KW - Macrophages
KW - P-selectin
KW - VCAM-1
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U2 - 10.1007/s12012-011-9140-0
DO - 10.1007/s12012-011-9140-0
M3 - Article
C2 - 21863403
AN - SCOPUS:84860395286
SN - 1530-7905
VL - 12
SP - 73
EP - 82
JO - Cardiovascular Toxicology
JF - Cardiovascular Toxicology
IS - 1
ER -