Subregion- and cell type-restricted gene knockout in mouse brain

Joe Z. Tsien, Dong Feng Chen, David Gerber, Cindy Tom, Eric H. Mercer, David J. Anderson, Mark Mayford, Eric R. Kandel, Susumu Tonegawa

Research output: Contribution to journalArticlepeer-review

975 Scopus citations


Using the phage P1-derived Cre/loxP recombination system, we have developed a method to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region. The Cre/loxP recombination-based gene deletion appears to require a certain level of Cre protein expression. The brain subregional restricted gene knockout should allow a more precise analysis of the impact of a gene mutation on animal behaviors.

Original languageEnglish (US)
Pages (from-to)1317-1326
Number of pages10
Issue number7
StatePublished - Dec 27 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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