Super-STORM: Molecular Modeling to Achieve Single-molecule Localization with STORM Microscopy

Zhichao Fan; Zbigniew Mikulski; Sara McArdle; Prithu Sundd; Klaus Ley

doi:10.1016/j.xpro.2019.100012

Super-STORM: Molecular Modeling to Achieve Single-molecule Localization with STORM Microscopy

Zhichao Fan, Zbigniew Mikulski, Sara McArdle, Prithu Sundd, Klaus Ley

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

This protocol introduces the SuperSTORM technique, combining stochastic optical reconstruction microscopy (STORM) and molecular modeling. SuperSTORM is optimized for acquiring and processing STORM images of neutrophil integrins but can be used for any cell-surface molecule with known structure and antibody-binding site(s). SuperSTORM identifies molecular cut-offs for eliminating multiple blinks of STORM imaging, determines colocalization, identifies clusters, and reveals molecular orientations and distributions. This protocol extends STORM imaging to cells in microfluidic systems. Improved resolution is achieved by using biomolecule-inherent parameters. For complete information on the generation and use of this protocol, please refer to the paper by Fan et al. (2019).

Original language	English (US)
Article number	100012
Journal	STAR Protocols
Volume	1
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2019.100012
State	Published - Jun 19 2020
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology
General Neuroscience

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10.1016/j.xpro.2019.100012

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abstract = "This protocol introduces the SuperSTORM technique, combining stochastic optical reconstruction microscopy (STORM) and molecular modeling. SuperSTORM is optimized for acquiring and processing STORM images of neutrophil integrins but can be used for any cell-surface molecule with known structure and antibody-binding site(s). SuperSTORM identifies molecular cut-offs for eliminating multiple blinks of STORM imaging, determines colocalization, identifies clusters, and reveals molecular orientations and distributions. This protocol extends STORM imaging to cells in microfluidic systems. Improved resolution is achieved by using biomolecule-inherent parameters. For complete information on the generation and use of this protocol, please refer to the paper by Fan et al. (2019).",

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Super-STORM: Molecular Modeling to Achieve Single-molecule Localization with STORM Microscopy

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