Surface-enhanced raman scattering of bacteria in microwells constructed from silver nanoparticles

Mustafa Çulha, M. Müge Yazici, Mehmet Kahraman, Fikrettin Şahin, Sesin Kocagöz

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


Whole bacterial cell characterization is critically important for fast bacterial identification. Surface-enhanced Raman scattering (SERS) is proven to be powerful technique to serve such a goal. In this study, the characterization of whole bacterial cells in the microwells constructed from colloidal silver nanoparticles (AgNPs) with convective-assembly method is reported. The proper size of the microwells for the model bacteria, Escherichia coli and Staphylococcus cohnii, is determined to be 1.2m from their electron microscopy images. A minimum dilution factor of 20 is necessary for the bacterial samples collected from growth media to diminish the bacterial aggregation to place the bacterial cells into the microwells. The constructed microwell structures are tested for their bacterial SERS performance and compared to the SERS spectra obtained from the samples prepared with a simple mixing of bacteria and AgNPs for the same bacteria. The results indicate that microwell structures not only improve the spectral quality but also increase the reproducibility of the SERS spectra.

Original languageEnglish (US)
Article number297560
JournalJournal of Nanotechnology
StatePublished - 2012
Externally publishedYes

ASJC Scopus subject areas

  • Materials Science(all)


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