TY - JOUR
T1 - Synchrotron radiation circular dichroism (SRCD) spectroscopy
T2 - An emerging method in structural biology for examining protein conformations and protein interactions
AU - Wallace, B. A.
AU - Gekko, Kunihiko
AU - Vrønning Hoffmann, Søren
AU - Lin, Yi Hung
AU - Sutherland, John C.
AU - Tao, Ye
AU - Wien, Frank
AU - Janes, Robert W.
N1 - Funding Information:
Supported by grants from the UK Biotechnology and Biological Sciences Research Council (to BAW and RWJ) and beamtime grants from the following synchrotrons: ISA, NSLS, BSRF, SRS, HiSOR, Diamond, SOLEIL, and NSRRC .
PY - 2011/9/1
Y1 - 2011/9/1
N2 - Circular dichroism (CD) spectroscopy is a well-established technique in structural biology. The use of synchrotron radiation as an intense light source for these measurements extends the applications possible using lab-based instruments. In recent years, there has been a major growth in synchrotron radiation circular dichroism (SRCD) beamlines worldwide, including ones at the NSLS, ISA, SRS, HiSOR, BSRF, NSRRC, SOLEIL, Diamond, TERAS, BESSYII, and ANKA synchrotrons. Through the coordinated efforts of beamline scientists and users at these sites, important proof-of-principle studies have been done enabling the method to be developed for novel and productive studies on biological systems. This paper describes the characteristics of SRCD beamlines and some of the new types of applications that have been undertaken using these beamlines.
AB - Circular dichroism (CD) spectroscopy is a well-established technique in structural biology. The use of synchrotron radiation as an intense light source for these measurements extends the applications possible using lab-based instruments. In recent years, there has been a major growth in synchrotron radiation circular dichroism (SRCD) beamlines worldwide, including ones at the NSLS, ISA, SRS, HiSOR, BSRF, NSRRC, SOLEIL, Diamond, TERAS, BESSYII, and ANKA synchrotrons. Through the coordinated efforts of beamline scientists and users at these sites, important proof-of-principle studies have been done enabling the method to be developed for novel and productive studies on biological systems. This paper describes the characteristics of SRCD beamlines and some of the new types of applications that have been undertaken using these beamlines.
KW - Conformational changes
KW - Macromolecular interactions
KW - Protein secondary structure
KW - Synchrotron radiation circular dichroism spectroscopy
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U2 - 10.1016/j.nima.2010.10.135
DO - 10.1016/j.nima.2010.10.135
M3 - Article
AN - SCOPUS:79961171352
SN - 0168-9002
VL - 649
SP - 177
EP - 178
JO - Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
JF - Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
IS - 1
ER -